OneStep qMethyl-PCR Kit

D5310 / D5311


OneStep qMethyl-PCR Kit

Cat # Name Size
D5310 OneStep qMethyl-PCR Kit Full Kit
D5311 OneStep qMethyl-Lite Lite
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Highlights
  • Single step, real-time PCR procedure for bisulfite-free determination of DNA methylation status
  • Includes reagents and controls for quantitative detection and reliable performance
  • Ideal for rapid screening of single and multi-locus DNA methylation
Description

The OneStep qMethyl Kit provides a simple, straightforward, and bisulfite-free procedure for rapid, locus-specific DNA methylation assessment via the selective amplification of a methylated region of DNA. This is accomplished by splitting any DNA to be tested into two parts: a “Test Reaction” and a “Reference Reaction”. DNA in the Test Reaction is digested with Methylation Sensitive Restriction Enzymes (MSREs) while DNA in the Reference Reaction is not. The DNA from both samples is then amplified using real-time PCR in the presence of SYTO 9 fluorescent dye and quantitated using a simple equation.

Input High quality DNA suitable for use in restriction enzyme digestion. Require A260/280 g&t; 1.8, A260/230 > 1.8.
Processing Time 4.5 hours
Storage Store at -20°C for up to 12 months. Avoid repeated freeze-thaw cycles of reagents. Prolong storage at -80°C. 2X Test Reaction PreMix and 2X Reference Reaction PreMix are light sensitive. Minimize light exposure.
Cat # Name Size
W1001-1 DNase/RNase-Free Water 1 ml
D5014 Human Methylated & Non-methylated DNA Set 5 µg/20 µl
D5311-1 2X Test Reaction-Lite PreMix Test Reaction
D5311-2 2X Reference Reaction-Lite PreMix Reference Reaction
D5310-1 2X Test Reaction PreMix Test Reaction
D5310-2 2X Reference Reaction PreMix Reference Reaction