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Total RNA Sequencing

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Compatible with low input, FFPE samples, and any organism (mammals, plants, bacteria, and more)
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Validated solution for unbiased gene expression profiles
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Comprehensive publication-ready bioinformatics gene expression analysis report
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User Friendly & Comprehensive Report

Publication-Ready Gene Expression Analysis at the Touch of a Button

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Full Analysis Includes:

  • Sample Distance Matrix (PCA/heatmap)
  • Differential gene expression
  • Scatter plot
  • MA plot
  • Gene set enrichment

Unbiased Gene Expression Data with Probe-Free Technology

Workflow Certified Using RNA Standards of Known Composition

Probe-Free rRNA Depletion
Unbiased Libraries
(264 genes affected)
Plot graph of Probe-Free rRNA depletion unbiased libraries
Probe-Based rRNA Removal
Biased Libraries
(3,603 genes affected)
Plot graph of Probe-Based rRNA removal biased libraries
Human reference RNA + ERCC spike-in samples subjected to Zymo-Seq probe-free rRNA depletion or competing methods shows dramatically biased transcript removal using Probe-based technique.

Robust Workflow Compatible with Any Sample Type

Graph of depletion comparison between human, mouse and e.coli
Compatible with many diverse sample types including human, mouse, bacteria, plant (A. thaliana, rat, whole blood, peripheral leukocytes, and more)!

microRNA Sequencing

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Efficient microRNA capture and isolation
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Compatible with cfRNA from plasma and serum samples
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Comprehensive publication-ready microRNA expression data analysis
View Sample Report

Efficient microRNA Capture from Biological Samples

Graph of percent algined to hg38
Graph of percent algined to miRBase
Total cell-free RNA from a set of three different donors captured using ZymoResearch RNA isolation methods followed by optimized cfRNA-seq library prep generates more reads that align to either the human reference genome (hg38, left/top) or a microRNA database (miRBase, right/bottom). View the white paper to learn more.

Total RNA-Seq Technical Specifications

Sequencing Platform NovaSeq 6000 or NovaSeq X
Read Length 2 x 150 bp, Unique Dual Indexing (UDI)
Sequencing Depth ~30 mln read pairs (can be customized)
Accepted Sample Types RNA, Cells, Tissue, FFPE Tissue Samples
Library Prep Zymo-Seq RiboFree Total RNA Library Kit (cat. R3000)
rRNA Depletion Enzymatic rRNA depletion compatible with any species
Data Visualization Sample similarity matrix, MDS plot, heatmap with top gene expression patterns, scatter plot, MA plot
Statistical Analysis Differential gene expression analysis with DESeq2 package, gene set entrichment analysis (if pathway annotation is available)

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