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    Quick-cfDNA/cfRNA Serum & Plasma Kit


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    Cat # Name Size
    R1072 Quick-cfDNA/RNA Serum & Plasma Kit 50 Preps

    Highlights

    • Quick & Easy: Simple spin-column based isolation from up to 3 mL of plasma, serum, or other biological liquids. No phenol/chloroform or precipitation.
    • Highest Yields: Efficient isolation of cell-free DNA and RNA. Recover up to 515 times more microRNA.
    • Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, nCounter, etc.
    Description & Documents Specifications Components

    Documents

    Product Description

    The Quick-cfDNA/cfRNA Serum & Plasma Kit enables simple and efficient preparation of all circulating cell-free DNA and RNA (including protein-bound, exosomal, microRNA, and other small RNA) from serum, plasma, and other biological fluids, without using toxic phenol-chloroform or cumbersome protein precipitation and removal steps. The kit efficiently separates cell-free DNA and RNA, with option to co-elute. Cell-free nucleic acids scale linearly relative to the sample input volume up to 3 ml. Zymo-Spin™ Technology enables isolation of ultra-pure cell-free nucleic acids suitable for all subsequent analyses, such as RT-qPCR and NGS.

    Documents

    Technical Specifications

    Elution Volume cfDNA and cfRNA can be eluted into 15 µl (to maximize total yield) or as little as 6 µl (for highly concentrated eluate) of DNase/RNase-Free Water.
    Processing Time Typically requires 30 minutes (cfDNA/cfRNA co-elution protocol) to 45 minutes (cfDNA/cfRNA separation protocol) hands-on time to process 10 samples. Sample digestion requires 2 hours.
    Purity High quality cell-free DNA (cfDNA) and cell-free RNA (cfRNA) are ready for all sensitive downstream applications such as RT-qPCR and Next-Generation Sequencing.
    Required Equipment Microcentrifuge, vortex, water bath or heating block, vacuum, and vacuum manifold.
    Sample Types Up to 3 ml of plasma, serum, cerebrospinal fluids, amniotic fluids, urine, or other biological fluids.
    Size DNA ≥ 50 bp and RNA ≥ 17 nt.
    Yield Yields are sample dependent and may vary. Typical recovery ranges from 1 – 100 ng/ml of human plasma or serum for both cfDNA and cfRNA. Kit is optimized for recovery of total cfDNA and cfRNA, including small RNA and microRNA.

    Resources

    Cat # Name Size
    R1072-1-150 Quick-cfDNA/cfRNA Digestion Buffer 150 mL
    R1072-2-150 Quick-cfDNA/cfRNA Binding Buffer 150 mL
    W1001-10 DNase/RNase-Free Water 10 ml
    R1003-3-12 RNA Wash Buffer 12 ml
    R1060-2-100 RNA Prep Buffer 100 ml
    R1072-3-20 Cell-free Recovery Buffer 20 ml
    C1004-50 Zymo-Spin IC Columns 50 Pack
    C1006-50-F Spin-Away Filters 50 Pack
    C1039-25 25 ml Reservoir for Spin-Away Filters and Zymo-Spin IIICG 25 Pack

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