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    Quick-DNA Plant/Seed Miniprep Kit


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    Cat # Name Size
    D6020 Quick-DNA Plant/Seed Miniprep Kit 50 Preps
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    Highlights

    • Rapid and simple method for DNA isolation from tough-to-lyse plant and seed samples.
    • Ultra-high density BashingBeads are fracture resistant and chemically inert.
    • Zymo-Spin column technology coupled with filtration removes polyphenolic PCR inhibitors from the DNA product.
    Description & Documents Specifications FAQ Components

    Documents

    Supplemental Info

    Product Description

    The Quick-DNA Plant/Seed Miniprep Kit is a plant DNA isolation kit designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in minutes: plant samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Polysaccharides, lipids, and polyphenols/tannins are removed from the DNA using our Zymo-Spin column technology. The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, etc.

    Documents

    Technical Specifications

    Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
    Elution Volume ≥ 50 µl
    Equipment Microcentrifuge, vortex, cell disruptor/pulverizer
    Processing Volume ≤ 150 mg
    Purity A260/A280 nm ≥1.8.
    Sample Source Leaves, stems, buds, flowers, fruit, seeds, etc.
    Sample Storage DNA stored at ≤ -20°C.
    Size Range Capable of recovering genomic DNA up to and above 40 kb. Typical fragment sizes range from 25 kb - 35 kb. If present, parasitic and viral DNA will also be recovered.
    Type Total DNA
    Yield ≤ 25 µg total DNA
    Supplemental Info

    Resources

    We currently do not have a list of plants, aside from what we have shown in the protocols: – A.thaliana – Juniper – Milkweed Leaf – Milkweed Leaflet – Milkweed Pre-Flowering Bud – Corn Kernel – Sunflower Seed – Nicotiana sp.

    A viscous sample can indicate incomplete sample lysis. Try using less of your sample and optimize bead beating conditions (duration, speed, time) to ensure samples are thoroughly lysed. After bead beating, pellet the cell debris before moving on. Adding more Genomic Lysis buffer to the lysate can help dilute and deproteinate the sample, making the sample less viscous and more suitable for DNA recovery.

    We have validated our kits with both high-speed homgenizers and low-speed disruptors. We highly recommend users to optimize their bead beating conditions for their own instruments. We recommend using a 2 ml-tube adapter to ensure that the bead beating is efficent (do not use adapters made of foam). For high-speed homogenizers, we recommend a total of 5 mins bead beating (1 min interval at 6.5 m/s with 5 mins rest, repeat 5 times). For low-speed cell disruptors, we recommend 30 mins at max speed.

    Environmental samples often contain inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanins, etc. that often co-purify and affect downstream applications such as PCR. The Zymo-Spin III-HRC column removes these polyphenolic PCR inhibitors to recover DNA that is ready for sensitive downstream applications such as Next-Gen Sequencing, quantitative PCR, etc.

    Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM). However, if bead beating is optimized and lysis is efficient, the addition of BME is not necessary and can be omitted.

    The Quick-DNA kits recover RNA-free genomic DNA. The selective chemistry allows for binding of double stranded DNA to the column and for RNA to flow through. No RNase A treatment is required when processing samples within kit capacity.

    Cat # Name Size
    D3004-1-100 Genomic Lysis Buffer 100 ml
    D3004-2-50 g-DNA Wash Buffer 50 ml
    D3004-4-10 DNA Elution Buffer 10 ml
    D3004-5-250 DNA Pre-Wash Buffer 250 ml
    D6001-3-40 BashingBead Buffer 40 ml
    D6035-1-30 Prep Solution 30 ml
    C1057-50 Zymo-Spin III-F Filters 50 Pack
    C1058-50 Zymo-Spin III-HRC Filters 50 Pack
    S6003-50 ZR BashingBead Lysis Tubes (2 mm) 50 Tubes
    C1078-50 Zymo-Spin IICR Columns 50 Pack

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