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    ZymoBIOMICS 96 MagBead DNA Kit


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    Cat # Name Size
    D4302 ZymoBIOMICS 96 MagBead DNA Kit (Lysis Rack) 2 x 96 Preps
    D4306 ZymoBIOMICS 96 MagBead DNA Kit (No Lysis Matrix) 2 x 96 Preps
    D4308 ZymoBIOMICS 96 MagBead DNA Kit (Lysis Tubes) 2 x 96 Preps
    D4302-E ZymoBIOMICS 96 MagBead DNA Kit – Dx (Lysis Rack) 2 x 96 Preps
    D4306-E ZymoBIOMICS 96 MagBead DNA Kit – Dx (No Lysis Matrix) 2 x 96 Preps
    D4308-E ZymoBIOMICS 96 MagBead DNA Kit – Dx (Lysis Tubes) 2 x 96 Preps

    Highlights

    • High-throughput purification of high quality, inhibitor-free DNA from any sample including feces, soil, water, biofilms, swabs, saliva, and body fluids.
    • The ZymoBIOMICS innovative lysis system enables efficient and unbiased lysis of microbes including Gram-positive/negative bacteria, fungi, protozoans, algae, etc.
    • The automation friendly workflow enables nearly any sample to be processed in as little as 90 minutes for 96 preps.
    Description & Documents Specifications FAQ Components

    Documents

    Supplemental Info

    Product Description

    The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.

    Learn More

    For automation scripts and support, email automation@zymoresearch.com

    Documents

    Technical Specifications

    Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
    Elution Volume ≥ 37.5 µl DNA Elution Buffer or DNase free water
    Equipment Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block
    Processing Volume Fecal ≤100 mg, Soil ≤ 200 mg, Cells ≤ 20 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells)
    Purity High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing
    Sample Source Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water.
    Sample Storage Eluted DNA should be stored at ≤ -20°C.
    Size Range Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.
    Type Total DNA
    Yield Up to 10 µg total DNA can be eluted into 50 µl (37.5 µl minimum)
    Supplemental Info

    Resources

    Yes, we currently have scripts for Hamilton and KingFisher, as well as support for Tecan on some systems. Please contact product specialist for specific inquiries.

    Yes, our protocol includes an “automation guide” for users to script the protocol themselves and our technical support staff also provides troubleshooting and advice if needed.

    Check sample type and input amount. Low biomass samples such as swabs, some soil samples, water, air are expected to have low yield. High biomass samples such as feces may cause overloading of the spin column. Using less input may improve yields.

    Ensure sample is fully homogenized. Depending on bead beater used, the settings may have to be optimized for each sample. The homogenization time may be extended to see if yields improve.

    Apply heated elution buffer (60-70° C) and allow elution buffer to incubate on the column for several minutes prior to elution.

    Ensure that the DNA Elution Buffer is added directly to the column matrix.

    Low A260/230 values can be due to handling issues (e.g. transfer of column during wash steps). To minimize reagent wash buffer carryover, centrifuge the column at max speed for 1 minute prior to elution.

    Cat # Name Size
    D4302-3-100 ZymoBIOMICS MagWash 1 100 ml
    D4300-1-150 ZymoBIOMICS Lysis Solution 150 ml
    D4302-4-200 ZymoBIOMICS MagWash 2 200 ml
    D4302-5-50 ZymoBIOMICS DNase/RNase Free Water 50 ml
    D4302-6-12 ZymoBIOMICS MagBinding Beads 12 ml
    S6012-50 ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) 50 Tubes
    S6002-96-3 ZR-96 BashingBead Lysis Rack 0.5 mm & 0.1 mm

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