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Format
Endura Tn5 Transposase - Unloaded
Highlights
- Exceptional Stability: Optimized novel formulation for superior stability.
- Batch-to-Batch Consistency: Enables highly reproducible assays and library preps.
- Ready-to-Load: Easily generate transposomes or load with custom adapters with Unloaded format.
Original Manufacturer
Satisfaction 100% guaranteed, read Our Promise
Innovated in California, Made in the USA
Format
Endura Tn5 Transposase - Unloaded
Highlights
- Exceptional Stability: Optimized novel formulation for superior stability.
- Batch-to-Batch Consistency: Enables highly reproducible assays and library preps.
- Ready-to-Load: Easily generate transposomes or load with custom adapters with Unloaded format.
Original Manufacturer
Satisfaction 100% guaranteed, read Our Promise
Innovated in California, Made in the USA
Description
Performance
Technical Specifications
| Activity | Tn5 Transposase (1 U/µl) |
|---|---|
| Format | Liquid, 50% glycerol |
| Concentration | ~12.5 µM, ~1 mg/ml |
| Optimum pH | 7.5 |
| Optimum Temperature | 55°C |
| Storage | Store at -20°C. For long term storage, store at -80°C. |
Resources
Documents
FAQ
Endura™ Tn5 Transposase is a hyperactive variant of Tn5 transposase with a novel formulation to confer superior stability. It can maintain its activity over 10 freeze-thaw cycles as well as room temperature incubation.
Endura™ Tn5 Transposase can be inactivated by addition of 10x Stop Buffer to a final concentration of 1x in your reaction, following by a 5-minute incubation at 75 °C.
Yes, you can load Endura™ Tn5 Transposase with any custom adapter sequence or transposon DNA, of any length, as long as the 3’ end contains 19-bp Mosaic-End sequence (5’ – CTGTCTCTTATACACATCT – 3’).
Applications
- Whole Genome Sequencing (WGS) – Decode the entire DNA sequence of an organism’s genome to identify genetic variants, mutations, and structural variations.
- Whole Exome Sequencing (WES) – Analyze the protein-coding regions of the genome to detect mutations linked to particular phenotypes, genetic disorders, and disease.
- Assay for Transposase-Accessible Chromatin Sequencing (ATAC-Seq) – Identify genome-wide chromatin accessibility and regulatory DNA elements in high resolution.
- Epigenomic Sequencing – Map DNA methylation and histone modifications to study gene expression regulation and epigenetic changes.
- Metagenomic Sequencing – Analyze microbial DNA from environmental or host-associated samples to profile microbial diversity, taxonomy, and functional genes.
- Single Cell Sequencing – Analyze gene expression, epigenetics, or genome variation at the individual cell level to uncover cellular heterogeneity.
- RNA Sequencing – Capture and quantify the transcriptome to analyze gene expression, alternative splicing, and non-coding RNA profiles.
- Transposome Studies – Examine transposable elements and mobile DNA to understand their role in genome evolution, stability, and regulation.
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