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    Quick-DNA Fecal/Soil Microbe Microprep


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    Cat # Name Size
    D6012 Quick-DNA Fecal/Soil Microbe Microprep 50 Preps

    Highlights

    • Rapid method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes, including those from humans, birds, rats, mice, cattle, etc.
    • Ultra-high density BashingBeads are fracture resistant and chemically inert.
    • Zymo-Spin column and unique filtration technologies effectively removes PCR inhibitors from the DNA product.
    Description & Documents Specifications FAQ Components

    Documents

    Supplemental Info

    Product Description

    The Quick-DNA Fecal/Soil Microbe Microprep Kit is a fecal and soil DNA extraction kit designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.

    Documents

    Technical Specifications

    Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
    Elution Volume ≥ 20 µl
    Equipment Microcentrifuge, vortex, cell disruptor/pulverizer
    Processing Volume ≤50 mg feces, ≤ 250 mg soil, ≤20 mg fungal/bacterial cells (wet weight)
    Purity A260/A280 nm ≥1.8.
    Sample Source Feces or soil
    Sample Storage DNA stored at ≤ -20°C.
    Size Range Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.
    Type Total DNA
    Yield ≤ 5 µg total DNA
    Supplemental Info

    Resources

    A viscous sample can indicate incomplete sample lysis. Try using less of your sample and optimize bead beating conditions (duration, speed, time) to ensure samples are thoroughly lysed. After bead beating, pellet the cell debris before moving on. Adding more Genomic Lysis buffer to the lysate can help dilute and deproteinate the sample, making the sample less viscous and more suitable for DNA recovery.

    We have validated our kits with both high-speed homgenizers and low-speed disruptors. We highly recommend users to optimize their bead beating conditions for their own instruments. We recommend using a 2 ml-tube adapter to ensure that the bead beating is efficent (do not use adapters made of foam). For high-speed homogenizers, we recommend a total of 5 mins bead beating (1 min interval at 6.5 m/s with 5 mins rest, repeat 5 times). For low-speed cell disruptors, we recommend 30 mins at max speed.

    Environmental samples often contain inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanins, etc. that affect downstream applications such as PCR. Once the DNA is eluted off the binding column, the DNA is then passed through the Zymo-Spin II-µHRC to remove the PCR inhibitors, and the DNA is then ready for downstream applications. The Zymo-Spin II-µHRC does not bind DNA, it simply removes the PCR inhibitors.

    Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM). However, if bead beating is optimized and lysis is efficient, the addition of BME is not necessary and can be omitted.

    No additional RNase A treatment is required when processing samples within kit capacity. The selective chemistry allows for binding of double stranded DNA to the column and for RNA to flow through.

    Cat # Name Size
    D3004-1-100 Genomic Lysis Buffer 100 ml
    D3004-2-50 g-DNA Wash Buffer 50 ml
    D3004-4-10 DNA Elution Buffer 10 ml
    D3004-5-15 DNA Pre-Wash Buffer 15 ml
    D6001-3-40 BashingBead Buffer 40 ml
    D6035-1-30 Prep Solution 30 ml
    C1057-50 Zymo-Spin III-F Filters 50 Pack
    C1004-50 Zymo-Spin IC Columns 50 Pack
    C1059-50 Zymo-Spin II-µHRC Filters 50 Pack
    S6012-50 ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) 50 Tubes

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