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Read The Zymo Research PromiseUniversal Methylated DNA Standard
| Cat # | Name | DNA Type |
|---|
Highlights
- Purified, methylated human DNA (D5011) and methylated mouse DNA (D5012) for use as controls to assess bisulfite conversion efficiency. Compatible with the EZ DNA Methylation family of products and other bisulfite conversion protocols.
- All cytosines within a CpG dinucleotide context have been enzymatically methylated by M. SssI methyltransferase.
- Each standard is provided with a primer set to amplify a fragment of DNA after bisulfite conversion.
Documents
Supplemental Info
Product Description
Technical Specifications
| Concentration | 250 ng/µl |
|---|---|
| Storage | Store DNA standard and primers at ≤ -20°C for up to 12 months. Prolonged storage should be ≤ -70°C. |
Resources
Q1: What is the source of the Universal Methylated Human DNA Standard (D5011) and Bisulfite-converted Universal Methylated Human DNA Standard (D5015)?
The sample is derived from human male brain tissue.
Q2: Are the Human methylated DNA standards completely (100%) methylated at the CpG sites?
All standards are enzymatically methylated by CpG Methylase in vitro at all CpG dinucleotides. The methylated DNA is very highly methylated (> 95% of all CpG sites).
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