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    ZR Plasmid Miniprep - Classic

    D4054 / D4015 / D4016

    ZR Plasmid Miniprep - Classic

    Cat # Name Size
    D4054 ZR Plasmid Miniprep - Classic 800 Preps
    D4015 ZR Plasmid Miniprep - Classic 100 Preps
    D4016 ZR Plasmid Miniprep - Classic 400 Preps



    • High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.
    • Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.
    • Unique column design: Zero buffer retention and low (30 µl) elution volume.

    The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.

    Applicable For Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, transfections, and other sensitive applications.
    Elution Volume ≥ 30 µl
    Endotoxin Levels < 50 EU/µg
    Equipment Microcentrifuge
    Processing Time 15 min
    Purity Typical Abs260/280 ≥1.8.
    Size Range Up to 25 kb
    Supplemental Info
    Yield Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.

    The ZymoPURE Miniprep kit utilizes a modified alkaline lysis system in conjunction with a novel binding system. This results in higher yields, higher concentrations, and lower endotoxin levels when compared to the ZR Plasmid Miniprep-Classic.

    No, we recommend using our ZR BAC DNA Miniprep Kit (D4048) or ZymoPURE Miniprep Kit for isolating large constructs.

    Yes, all our plasmid preps have an endo-wash step to remove endotoxins and produce plasmid DNA that is ready for transfection with most cell lines.

    10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.

    Yes, but we recommend performing a dry spin in the microcentrifuge after the last wash step, prior to the elution step.

    Plasmid Wash Buffer is available for purchase, separately. However, you can substitute with DNA Wash Buffer, Zyppy Wash Buffer, or 80% ethanol.

    ≤ 50 Endotoxin Units/µg of plasmid DNA.

    Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4 °C as soon as possible to ensure optimal performance throughout the life span of the product.

    Cat # Name Size
    D3004-4-16 DNA Elution Buffer 16 ml
    D4036-3-60 Endo-Wash Buffer 60 ml
    D4036-3-30 Endo-Wash Buffer 30 ml
    D3004-4-4 DNA Elution Buffer 4 ml
    D4027-1-160 Buffer P1 (Red) 160 ml
    D4027-1-20 Buffer P1 (Red) 20 ml
    D4027-1-80 Buffer P1 (Red) 80 ml
    D4027-2-160 Buffer P2 (Green) 160 ml
    D4027-2-20 Buffer P2 (Green) 20 ml
    D4027-2-80 Buffer P2 (Green) 80 ml
    D4027-3-220 Buffer P3 (Yellow) 220 ml
    D4027-3-50 Buffer P3 (Yellow) 50 ml
    D4027-4-24 Plasmid Wash Buffer 24 ml
    D4027-4-48 Plasmid Wash Buffer 48 ml
    D4027-4-6 Plasmid Wash Buffer 6 ml
    C1001-50 Collection Tubes 50 Pack
    C1019-50 Zymo-Spin IIN Columns 50 Pack

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