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    Zymoclean Gel RNA Recovery Kit


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    Cat # Name Size
    R1011 Zymoclean Gel RNA Recovery Kit 50 Preps

    Highlights

    • Quick and Reliable: 30 minute recovery of purified RNA fragments from agarose gels.
    • Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
    • High Recovery: ≥ 80% recovery for RNA > 500 nt.
    Description & Documents Specifications FAQ Components Citations

    Documents

    Product Description

    The Zymoclean Gel RNA Recovery Kit provides a quick and efficient purification method for recovery of RNA fragments from agarose gels. The procedure combines a unique, single-step agarose dissolving/RNA binding buffer with Zymo-Spin column technology to yield high quality, purified RNA in just minutes. The purified RNA is eluted into small volumes of DNase/RNase-free water for highly concentrated samples suitable for subsequent RNA-based manipulations. Compatible with MOPS, TAE, and TBE buffered agarose gels (formaldehyde up to 2.0%).

    Documents

    Technical Specifications

    Equipment Microcentrifuge, 37°C-65°C heat source
    Purity RNA is ready for all subsequent analysis and molecular manipulation
    Sample Source Single- or double-stranded RNA fragments (≥200 nt) resolved in TAE TBE, and MOPS buffered agarose gels. Compatible with formaldehyde to 2.0% (final conc.)
    Size Range RNA fragments ≥500 nt (≥80% recovery)
    Yield 10 µg RNA (binding capacity), ≥6 µl (elution volume)

    Resources

    Yes, the Zymo-Spin IC Columns and RNA Wash Buffer are interchangeable.

    No, it’s not optimized for samples in solution. High efficiency recovery is not guaranteed and the success of the purification can be variable.

    Up to 400 mg of agarose.

    Yes. Use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.

    Yes, the kit efficiently removes unincorporated fluorescent dyes, radiolabeled dNTP’s and Biotin.

    Cat # Name Size
    W1001-1 DNase/RNase-Free Water 1 ml
    R1003-3-12 RNA Wash Buffer 12 ml
    R1011-1-50 RAD Buffer (RNA Agarose Dissolving Buffer) 50 ml
    R1060-2-25 RNA Prep Buffer 25 ml
    C1001-50 Collection Tubes 50 Pack
    C1004-50 Zymo-Spin IC Columns 50 Pack

    Dimeric RNA was extracted from gel slice using the Zymoclean Gel RNA Recovery kit for Northern analysis of signal recognition particle (SRP) RNA.

    Rulli Jr SJ, et al. Selective and Nonselective Packaging of Cellular RNAs in Retrovirus Particles. Journal of Virology. 2007.

    The Zymoclean Gel RNA Recovery kit was used on agarose gels with RNA derived from Candida albicans strains. Researchers showed new mechanisms that regulates rRNA processing involving 18S, 5.8S and 25S rRNAs.

    Pendrak, ML et al. Ribosomal RNA processing in Candida albicans. RNA Journal. 2011.

    A dsRNA full-length transcript standard was visualized by electrophoresis on a 1% denaturing agarose gel and isolated using the Zymoclean Gel RNA Recovery kit. This rotavirus (RVA) dsRNA was used in qRT-PCR for RVA detection with the limit of detection being 1 genome copy per reaction.

    Mijatovic-Rustempasic S et al. Sensitive and Specific Quantitative Detection of Rotavirus A by One-Step Real-Time Reverse Transcription-PCR Assay without Antecedent Double-Stranded-RNA Denaturation. Journal of Clinical Microbiology. 2013.

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