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    ZymoPURE Plasmid Miniprep Kit


    Zymo Research's 100% Satisfaction Guarantee

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    Read The Zymo Research Promise

    Cat # Name Size
    D4212 ZymoPURE Plasmid Miniprep Kit 800 Preps
    D4211 ZymoPURE Plasmid Miniprep Kit 400 Preps
    D4210 ZymoPURE Plasmid Miniprep Kit 100 Preps
    D4209 ZymoPURE Plasmid Miniprep Kit 50 Preps
    D4208T ZymoPURE Plasmid Miniprep Kit 10 Preps
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    Highlights

    • Highest Yield: Purify up to 100 ”g of plasmid DNA in as little as 25 ”l directly from a spin-column.
    • Transfection-Grade: 50,000 times fewer endotoxins than industry leading minipreps.
    • BAC/YAC/PAC Ready: Purify DNA up to ~200 kb.
    Description & Documents Specifications FAQ Components

    Documents

    Supplemental Info

    Product Description

    Part of the ZymoPUREℱ Plasmid Kits collection, the ZymoPUREℱ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 ”g of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The unique spin-column design also provides zero buffer retention and a low elution volume. ZymoPUREℱ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 ”g/”l). In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications. As an added convenience, the ZymoPUREℱ Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.

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    Documents

    Technical Specifications

    Applicable For Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications.
    Binding Capacity 100 µg
    Culture Input ≤ 5 mL
    Elution Volume 25 µl
    Endotoxin Levels ≤ 1 EU/µg
    Equipment Microcentrifuge and/or vacuum manifold
    Format Spin Column
    Processing Time 15 min
    Purity Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0
    Recommended Sample Volume ≤ 5.0 ml
    Size Range Up to ~200 kb
    Yield Up to 100 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized.
    Supplemental Info

    Resources

    10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.

    No, the ZymoPURE™ kits are only compatible with ZymoPURE™ Wash 2 and we cannot disclose a substitution due to the sensitive nature of the recipe. Additional ZymoPURE™ Wash 2 can be purchased separately.

    The vacuum pump should be a single or double-staged unit capable of producing at least 400 mm Hg pressure at the vacuum manifold. If less pressure is applied, centrifuge the column prior to washing to remove any residual lysate/buffer remaining in the matrix.

    Yes, any vacuum that uses standard luer-lock connectors is compatible.

    Yes, however, the pressure needs to be around 400 mm Hg. Users should take caution, as the pressure of an in-house vacuum line can fluctuate drastically or be significantly reduced, depending on the demand in the building.

    Yes, please contact technical support for an application note.

    Yes, the low-copy protocol can be found in the Appendix of the kit protocol.

    Yes, however, more attention is required during the lysis and steps. We recommend centrifuging/pelleting the neutralized lysate before loading onto the supplied syringe/bottle-top filter. For high copy plasmids using more culture can cause overloading and subsequent clogging of columns, which can result in poor DNA yield and/or quality.

    Yes, the standard ZymoPURE protocol has been successfully tested with constructs up to 200 kb. To increase elution of large plasmid DNA, we recommend pre-warming the ZymoPURE Elution Buffer (50 ÂșC) and increasing the incubation time on column up to 10 minutes prior to centrifugation.

    Yes, the standard protocol should work with other gram-negative bacteria. However, the user will need to validate the protocol for their particular species. For gram-positive bacteria, please refer to the Gram-Positive Bacteria Protocol in the Appendix of the kit protocol.

    Cat # Name Size
    C1086-50 Zymo-Spin II-PX Columns 50 Pack
    C1001-50 Collection Tubes 50 Pack
    D4200-6-23 ZymoPURE Wash 2 (Concentrate) 23 ml
    D4200-4-3 ZymoPURE Binding Buffer 3 ml
    D4200-5-20 ZymoPURE Wash 1 20 ml
    D4200-5-320 ZymoPURE Wash 1 320 ml
    D4200-7-6 ZymoPURE Elution Buffer 6 ml
    D4200-6-28 ZymoPURE Wash 2 (Concentrate) 28 ml
    D4200-7-12 ZymoPURE Elution Buffer 12 ml
    D4200-3-210 ZymoPURE P3 (Yellow) 210 ml
    D4200-3-3 ZymoPURE P3 (Yellow) 3 ml
    D4200-4-110 ZymoPURE Binding Buffer 110 ml
    D4200-4-14 ZymoPURE Binding Buffer 14 ml
    D4200-2-210 ZymoPURE P2 (Blue) 210 ml
    D4200-2-3 ZymoPURE P2 (Blue) 3 ml
    D4200-3-100 ZymoPURE P3 (Yellow) 100 ml
    D4200-3-13 ZymoPURE P3 (Yellow) 13 ml
    D4200-1-210 ZymoPURE P1 (Red) 210 ml
    D4200-1-3 ZymoPURE P1 (Red) 3 ml
    D4200-2-100 ZymoPURE P2 (Blue) 100 ml
    D4200-2-13 ZymoPURE P2 (Blue) 13 ml
    D4200-1-100 ZymoPURE P1 (Red) 100 ml
    D4200-1-13 ZymoPURE P1 (Red) 13 ml
    D4200-7-30 ZymoPURE Elution Buffer 30 ml

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