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    Quick-RNA Whole Blood


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    Cat # Name Size
    R1201 Quick-RNA Whole Blood 50 preps

    Highlights

    • Superior Yields: Recover total RNA (including small/micro RNAs) without sample loss.
    • Protection: Worry-free blood sample storage at ambient temperatures for up to 30 days.
    • High-Quality: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-PCR, etc. DNase I Included.
    Description & Documents Specifications FAQ Components Citations

    Documents

    Product Description

    The Quick-RNA Whole Blood kit utilizes DNA/RNA Shield™, a unique preservation and lysis technology, to enable rapid isolation of total RNA from whole or partitioned blood or a cell pellet (after red blood cell lysis). The procedure uses Zymo-Spin column technology in which the sample is pre-filtered on the Zymo-Spin IIICG Column and then purified and concentrated on the Zymo-Spin IC Column. RNA is eluted into ≥ 6 µl of RNase-free water and is ready for any downstream application including RT-PCR, sequencing, etc.

    Documents

    Technical Specifications

    Equipment Microcentrifuge, vortex
    Purity RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
    Sample Source Up to 1 ml mammalian whole-blood, plasma, or serum. Also compatible with pelleted blood cells (PBMCs, WBCs, buffy coat, pelleted sample from PAXgene™ Blood RNA Tube, etc.) and nucleated blood.
    Size Range Total RNA ≥ 17 nt
    Yield 10 µg RNA (binding capacity), ≥6 µl (elution volume)

    Resources

    This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.

    Cat # Name Size
    W1001-4 DNase/RNase-Free Water 4 ml
    R1200-25 DNA/RNA Shield (2X Concentrate) 25 ml
    C1006-50-G Zymo-Spin IIICG Columns 50 Pack
    C1004-50 Zymo-Spin IC Columns 50 Pack
    C1001-50 Collection Tubes 50 Pack
    R1070-1-10 RNA Recovery Buffer 10 ml
    R1200-1-5 PK Digestion Buffer 5 ml
    R1060-2-25 RNA Prep Buffer 25 ml
    R1003-3-24 RNA Wash Buffer 24 ml
    E1010-1-4 DNA Digestion Buffer 4 mL

    RNA was extracted from canine blood using the Quick-RNA Whole Blood kit and used for PCR amplification for cloning canine TREM-1. TREM-1 expression was shown to be an amplifier or pro-inflammatory responses and has potential to be a biomarker for infection and pneumonia.

    Li, J et al. Expression and function of triggering receptor expressed on myeloid cells-1 (TREM-1) on canine neutrophils. Developmental and Comparative Immunology. 2011.

    The Quick-RNA Whole Blood kit was used to purify 200 ul human blood and RNA was used to generate cDNA for cloning. Data suggests that the SNPs of aminopeptidase ERAP1 can encode for different normal, hypo-, or hyper- functional activities.

    Reeves, E et al. Naturally Occurring ERAP1 Haplotypes Encode Functionally Distinct Alleles with Fine Substrate Specificity. The Journal of Immunology. 2013.

    HCV RNA was extracted from sera of human patients using the Quick-RNA Whole Blood kit and used for amplification and Roche 454 pyrosequencing. Results reveal that more genotype 1a isolates were associated with resistance to protease inhibitors.

    Margeridon-Thermet, S et al. Similar Prevalence of Low-Abundance Drug-Resistant Variants in Treatment-Naïve Patients with Genotype 1a and 1b Hepatitis C Virus Infections as Determined by Ultradeep Pyrosequencing. PLoS ONE. 2014

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