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    DNA Clean & Concentrator-100

    D4029 / D4030


    DNA Clean & Concentrator-100

    Cat # Name Size
    D4029 DNA Clean & Concentrator-100 25 Preps
    D4030 DNA Clean & Concentrator-100 50 Preps

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    Highlights

    • Simple, rapid recovery of ultra-pure DNA from PCR, endonuclease digestions, and cell-free DNA preps, etc.
    • Unique column construction allows sample loading and washing to be performed using a centrifuge, microcentrifuge, or vacuum source.
    • Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
    Description

    The DNA Clean & Concentrator-100 (DCC-100) is a PCR purification kit designed for the rapid desalting and purification of up to 100 µg of high quality DNA from PCR, large format restriction endonuclease digestions, or cell-free lysates. Eluted DNA is suitable for nucleotide sequencing, array analysis, PCR, nucleotide blotting, restriction endonuclease digestion procedures, as well as many other downstream applications requiring high quality DNA. The entire DNA purification/concentration procedure typically takes less than 20 minutes and can be performed using a syringe, centrifuge or vacuum source together with a microcentrifuge.


    Detergent Tolerance ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS
    Elution Volume ≥ 150 µl
    Equipment Microcentrifuge and centrifuge or vacuum source.
    Purity Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions.
    Sample Source DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.
    Size Range 50 bp to 23 kb
    Yield ≤ 100 µg total DNA can be recovered. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%.

    Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.

    Add an equal volume of ethanol (95-100%) to the sample and mix well. The sample is ready-to-bind and does not require DNA Binding Buffer. Proceed to Step 1.

    The DNA will be eluted off the column. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.

    Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.

    We recommend no more than 5 times as binding efficiency might decrease.

    Working with volumes below 50 µl can result in decreased recovery. We recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions.




    “This kit performed as described. I was able to obtain good quality and yield of the DNA -better than with a couple of competitor's kits.”

    - Kathleen B. (SUNY-ESF)

    “The simple steps and washing instructions were excellent. DNA was ready for use for PCR and gave strong results.”

    - Tyler C.

    “It was a very simple procedure and it gave a concentration ten times the original amount.”

    - Kimberly M. (University of Pennsylvania)

    Read More

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