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    ZR-96 DNA Sequencing Clean-up Kit


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    Cat # Name Size
    D4052 ZR-96 DNA Sequencing Clean-up Kit 2 x 96 Preps
    D4053 ZR-96 DNA Sequencing Clean-up Kit 4 x 96 Preps

    Highlights

    • Complete elimination of "dye blobs" for high quality Phred scores and long read lengths
    • Flexible 6-20 µl elution volumes allow for direct loading of samples with no precipitation or drying steps
    • Reusable!
    Description & Documents Specifications Components

    Documents

    Product Description

    The ZR-96 DNA Sequencing Clean-Up Kits provides a simple and rapid (2 & 10 minute) method for removal of post-cycle sequencing reaction contaminants (i.e., unincorporated fluorescent dyes, residual salts, dNTPs, primers, and enzymes) from DNA extension products. These contaminants can often interfere with the quality and signal strength of sequencing data, including dye peaks or "dye blobs" which may obscure portions of the sequencing chromatogram and interfere with base-calling accuracy of sequencing analysis software. DNA is eluted with a small volume of water or loading dye containing formamide.

    Documents

    Technical Specifications

    Elution Volume ≥ 15 µl of DNA Elution Buffer
    Equipment Centrifuge with microplate carriers
    Sample Source Sequencing input samples with post-cycle sequencing reaction contaminants from DNA extension products
    Sample storage Eluted DNA can be used immediately or stored at ≤ -20°C
    Yield Up to 5 µg DNA

    Resources

    Cat # Name Size
    D4050-1-55 Sequencing Binding Buffer 55 ml
    D4050-2-70 Sequencing Wash Buffer 70 ml
    C2008 96-Well PCR Conversion Plate 2 Plates
    C2002 Collection Plate 2 Plates
    C2006 Zymo-Spin IB-96 Plate 2 Plates

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