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    ZR-96 DNA Sequencing Clean-up Kit

    High-throughput and rapid clean up of dye terminator sequencing reactions prior to Sanger Sequencing.

    Highlights

    • Complete elimination of "dye blobs" for high quality Phred scores and long read lengths
    • Flexible 6-20 µl elution volumes allow for direct loading of samples with no precipitation or drying steps
    • Reusable!
    Zymo Research's 100% Satisfaction Guarantee

    Original Manufacturer

    Innovated in California, Made in the USA

    Satisfaction 100% guaranteed, read Our Promise

    Highlights

    • Complete elimination of "dye blobs" for high quality Phred scores and long read lengths
    • Flexible 6-20 µl elution volumes allow for direct loading of samples with no precipitation or drying steps
    • Reusable!
    Zymo Research's 100% Satisfaction Guarantee

    Original Manufacturer

    Innovated in California, Made in the USA

    Satisfaction 100% guaranteed, read Our Promise

    Cat # Name Size
    D4052 ZR-96 DNA Sequencing Clean-up Kit 2 x 96 Preps
    D4053 ZR-96 DNA Sequencing Clean-up Kit 4 x 96 Preps
    Cat # Name Size
    D4050-1-55 Sequencing Binding Buffer 55 ml
    D4050-2-70 Sequencing Wash Buffer 70 ml
    C2008 96-Well PCR Conversion Plate 2 Plates
    C2002 Collection Plate 2 Plates
    C2006 Zymo-Spin IB-96 Plate 2 Plates

    Description

    The ZR-96 DNA Sequencing Clean-Up Kits provides a simple and rapid (2 & 10 minute) method for removal of post-cycle sequencing reaction contaminants (i.e., unincorporated fluorescent dyes, residual salts, dNTPs, primers, and enzymes) from DNA extension products. These contaminants can often interfere with the quality and signal strength of sequencing data, including dye peaks or "dye blobs" which may obscure portions of the sequencing chromatogram and interfere with base-calling accuracy of sequencing analysis software. DNA is eluted with a small volume of water or loading dye containing formamide.

    Performance

    Technical Specifications
    Elution Volume ≥ 15 µl of DNA Elution Buffer
    Equipment Centrifuge with microplate carriers
    Sample Source Sequencing input samples with post-cycle sequencing reaction contaminants from DNA extension products
    Sample storage Eluted DNA can be used immediately or stored at ≤ -20°C
    Yield Up to 5 µg DNA

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