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    EZ-96 DNA Methylation-Lightning MagPrep

    Highlights

    • High-throughput procedure for automated rapid and complete bisulfite conversion of DNA for methylation analysis.
    • Ready-to-use conversion reagent is added directly to DNA.
    • High-yield, converted DNA is ideal for PCR, Methylation Specific PCR (MSP), arrays, library preps, Next-Generation sequencing, etc.
    Zymo Research's 100% Satisfaction Guarantee

    Original Manufacturer

    Innovated in California, Made in the USA

    Satisfaction 100% guaranteed, read Our Promise

    Highlights

    • High-throughput procedure for automated rapid and complete bisulfite conversion of DNA for methylation analysis.
    • Ready-to-use conversion reagent is added directly to DNA.
    • High-yield, converted DNA is ideal for PCR, Methylation Specific PCR (MSP), arrays, library preps, Next-Generation sequencing, etc.
    Zymo Research's 100% Satisfaction Guarantee

    Original Manufacturer

    Innovated in California, Made in the USA

    Satisfaction 100% guaranteed, read Our Promise

    Cat # Name Size
    D5046 EZ-96 DNA Methylation-Lightning MagPrep 4 x 96 Rxns.
    D5047 EZ-96 DNA Methylation-Lightning MagPrep 8 x 96 Rxns.
    D5046-E EZ-96 DNA Methylation Lightning MagPrep 4 x 96 Rxns.
    D5047-E EZ-96 DNA Methylation Lightning MagPrep 8 x 96 Rxns.
    Cat # Name Size
    D4100-5-8 MagBinding Beads 8 mL
    D4100-5-16 MagBinding Beads 16 mL
    D5041-6 M-Elution Buffer 40 ml
    D5007-6 M-Elution Buffer 8 ml
    D5040-4 M-Wash Buffer 72 ml
    D5040-3 M-Binding Buffer 250 ml
    D5032-1 Lightning Conversion Reagent 15 ml
    D5046-5 L-Desulphonation Buffer 80 ml
    C2005 Conversion Plate w/ Cover Foil 2 Plates/Foils
    C2002 Collection Plate 2 Plates
    C2003 Elution Plate 2 Plates

    Description

    The EZ-96 DNA Methylation-Lightning MagPrep Kit is the fastest magnetic bead-based DNA bisulfite conversion kit for high-throughput and automated methylation analysis. No preparation is necessary with the ready-to-use conversion reagent - simply add this unique reagent to a DNA sample and incubate the reaction for about one hour. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. This magnetic bead-based bisulfite conversion kit produces the highest yields of bisulfite-converted DNA that is ideal for PCR, arrays, library preps, and Next-Generation sequencing.

    For automation scripts and support, email automation@zymoresearch.com

    Performance

    Technical Specifications
    Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.
    Conversion > 99.5%
    Elution Volume ≥ 25 µl
    Equipment Thermocycler with heated lid, heating element for 96-well plate, magnetic stand.
    Input 100 pg - 2 µg of DNA.
    Processing Time 1.5 hours
    Recovery > 80%
    Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.

    Resources

    Documents

    FAQ

    Leaving the desulphonation buffer on the column longer than recommended will cause more degradation and subsequently result in lower yields.

    Bisulfite conversion will work regardless of context, so the kits are compatible with genomic DNA derived from plants and other species with high non-CpG methylation levels.

    ZymoTaq DNA Polymerase has been specifically designed for use in bisulfite amplification reactions. ZymoTaq is available as a stand-alone polymerase (E2001/E2002), PreMix (E2003/E2004), and qPCR PreMix (E2054/E2055).

    > 50 bp.

    For best results, keep the method of quantification consistent before and after bisulfite treatment:

    • If quantifying with a NanoDrop, use dsDNA settings (50 μg/ml for Ab260 = 1.0) before treatment and use RNA settings (40 μg/ml for Ab260 = 1.0) after treatment.
    • If quantifying with Qubit, use a dsDNA assay before treatment and use a ssDNA assay after treatment.
    Following bisulfite treatment of DNA, nonmethylated cytosine residues are converted into uracil. The recovered DNA is typically A, U, and T-rich. The original base-pairing no longer exists. Instead, the converted DNA is single stranded with limited non-specific base-pairing at room temperature. Therefore, traditional dsDNA methods of quantification will not accurately represent the amount of recovered bisulfite converted DNA.

    Following bisulfite treatment, DNA will be single stranded with limited non-specific base pairing at room temperature. To visualize, run the converted DNA on an agarose gel then chill the gel on ice or in an ice bath for 30 minutes. This will force enough base-pairing to allow intercalation of the ethidium bromide for the DNA to be visible. If using a Bioanalyzer or TapeStation instrument, use RNA kits and reagents to visualize the converted DNA.

    Poor conversion efficiency and low yields can be due to a variety of different experiment-specific conditions. Please contact Technical Support to discuss your specific experimental conditions and further troubleshoot with a product specialist.

    Converted DNA eluted in M-Elution Buffer can generally be stored at -20°C for 1-3 months. If longer term storage is necessary, we recommend storing at or below -70°C if possible. Bisulfite converted DNA is less stable than dsDNA; for best results, minimize freeze-thawing of converted DNA and use as soon as possible for downstream analysis.

    Product Video
    Reviews

    "It combines the speed of the lightning kit with the use of magnetic beads. This allows the full automation of bisulfite treatment. This is what we were looking for."

    Marco M.

    UCLA

    Citations

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