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    Quick-DNA Midiprep Plus Kit


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    Cat # Name Size
    D4075 Quick-DNA Midiprep Plus Kit 25 Preps

    Highlights

    • Extract high-quality DNA easily and reliably from any biological fluids, cultured/monolayer cells, or solid tissues.
    • Zymo-Spin Technology ensures DNA is ready for all sensitive downstream applications such as qPCR, DNA-sequencing, arrays, and methylation analysis.
    Description & Documents Specifications FAQ Components

    Documents

    Supplemental Info

    Product Description

    The Quick-DNA Plus Kits are the easiest DNA isolation kits for high yield total DNA extraction (e.g., genomic, mitochondrial, viral), cell culture, solid tissue, saliva, and any biological fluid sample. Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA > 50 kb to be eluted in as little as 10 µl. Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free bypassing the need for RNase A treatment and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.

    Documents

    Technical Specifications

    Elution Volume ≤ 200 µl
    Equipment Water bath or heat block (55°C), centrifuge or vacuum source and manifold, microcentrifuge, vortex, conical tubes (15 – 50 ml), and microcentrifuge tubes.
    Purity High quality DNA is ready for all sensitive downstream applications such as PCR, endonuclease digestion, Southern blotting, genotyping, Next-Gen sequencing, bisulfite conversion, etc. (A260/A230≥ 2.0).
    Size Range Capable of recovering genomic and mitochondrial DNA sized fragments > 50 kb. If present, parasitic, microbial, and viral DNA will also be recovered.
    Workflow Utilize a Proteinase K Digestion and Zymo-Spin Column for effective recovery of DNA.
    Yield The DNA binding capacity of the column is 125 µg. Typically, mammalian tissues yield: 1-3 µg DNA per mg skeletal, heart, lung, and brain tissues and 3-5 µg DNA per mg liver and kidney. Human whole blood will yield 3-7 µg DNA per 100 µl blood sampled.
    Supplemental Info

    Resources

    The BioFluid & Cell Buffer (Red) is designed to allow for rapid Proteinase K digestion with easy-to-lyse samples (e.g. mammalian cells and biological fluids). The Solid Tissue buffer (Blue) can be used for any sample type and requires a longer PK digestion time compared to using the BioFluid & Cell Buffer (Red).

    Yes, add 4 volumes of Genomic Lysis Buffer to 1 volume of crude lysate, homogenized, or digested sample (see Cell Suspensions and Proteinase K Digested Samples) and proceed with the remainder of the protocol.

    Beta-mercaptoethanol is a reducing agent that helps break down proteins and improves DNA recovery and purity. Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM) or omitted.

    No, the kit is designed for direct use with biological samples. For clean-up of previously isolated DNA, please use the Genomic DNA Clean & Concentrator or the DNA Clean & Concentrator kits.

    Keep in mind that there is sample to sample variability.

    Sample Type Input Amount Expected Yield
    Eukaryotic Cells 1x106 Cells 5-6 µg
    Skeletal, Heart, Lung, Brain Tissue 1 mg 1-3 µg
    Liver and Kidney Tissue 1 mg 3-5 µg
    Human Whole Blood 100 µl 5-7 µg

    White blood cells, which are the major source of genomic DNA in blood, easily and quickly settles. Mix the blood sample well prior to aliquoting for purification.

    Yes, samples can be digested overnight. Make sure to follow the appendix (page 8 appendix B) for processing liquids samples in DNA/RNA Shield and incubate at room temperature.

    The Quick-DNA is optimized for cells, soft tissues, and homogenized/digested samples using a single lysis/binding buffer. The Quick-DNA Plus kits contain an optimized Proteinase K for processing a wider variety of sample inputs, such as cells, blood, tissues, etc. The upgraded Quick-DNA Plus recovers more DNA with higher purity compared to the Quick-DNA Kits.

    E.coli cells are easy-to-lyse and can be processed directly with the Biological Fluids & Cells protocol. For an all-inclusive kit with any type of microbes (including tough-to-lyse), use any of Zymo Research’s Environmental Kits (e.g. Quick-DNA Fungal/Bacterial, Quick-DNA Fecal/Soil, ZymoBIOMICS DNA, etc.).

    Cat # Name Size
    C1001-50 Collection Tubes 50 Pack
    C1029-25 Zymo-Spin V-E Columns w/ 15 ml Conical Reservoir 25 Pack
    D3004-2-200 g-DNA Wash Buffer 200 ml
    D3004-5-250 DNA Pre-Wash Buffer 250 ml
    D4068-2-22 Solid Tissue Buffer (Blue) 22 ml
    D4068-1-45 BioFluid & Cell Buffer (Red) 45 ml
    D3004-4-10 DNA Elution Buffer 10 ml
    D3004-1-150 Genomic Lysis Buffer 150 ml
    D3001-2-20 Proteinase K w/ Storage Buffer Set 20 mg

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