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    Quick-DNA/RNA Microprep Plus Kit


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    Cat # Name Size
    D7005 Quick-DNA/RNA Microprep Plus Kit 50 Preps
    D7005T Quick-DNA/RNA Microprep Plus Kit 10 preps

    Highlights

    • Quick & Easy: Extract DNA and RNA from any sample including cells, solid tissue, whole blood, biological liquids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples), etc.
    • Sensitive: Single cell-level recovery of DNA and RNA.
    • Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, arrays, etc. DNase I included (Plus kit and MagBead only)
    Description & Documents Specifications FAQ Components

    Documents

    Product Description

    The Quick-DNA/RNA Microprep Plus kit combines Quick-DNA/RNA technology with the addition of DNA/RNA Shield, a unique preservation and lysis technology, and Proteinase K to enable easy, reliable, and rapid isolation from a wide range of sample sources, including cells, solid tissue, and whole blood. The procedure uses Zymo-Spin column technology that results in high-quality gDNA and total RNA, ready for any downstream application including reverse transcription, microarray, sequencing.

    Documents

    Technical Specifications

    Equipment Microcentrifuge, vortex, and heat block/bath (55°C)
    Purity DNA and RNA are ready for Next-Gen Sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
    Sample Source Any cells, solid tissue, whole blood, biological fluids, FFPE tissue, and samples stored in DNA/RNA Shield, etc.
    Size Range Genomic DNA ≥40 kb and Total RNA ≥17 nt
    Yield ≤ 5 µg DNA and ≤ 10 µg RNA

    Resources

    Yes, the catalog number for the DNase I set (DNase and DNA Digestion Buffer) that we offer is E1010.

    The kit is optimized with Zymo Research's DNase, however DNase from other manufacturers can be used. Follow the respective protocol for on-column DNase treatment. If the DNase does not have a protocol, proceed with in-tube DNase treatment post-extraction, then purify using the RNA Clean & Concentrator-5.

    Aliquot and store at – 20°C. Minimize multiple freeze thaw cycles. Lyophilized DNase I is stable at room temperature.

    Yes, the Quick-DNA/RNA MicroPrep Plus (#D7005) is capable of isolating from single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)

    If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.

    Yes, proteins can be precipitated. Please refer to the protocol appendix.

    Yes. Use 80% ethanol as a substitute. DNA/RNA Wash Buffer is also sold separately.

    Yes, both DNA and RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, qPCR, etc.

    Yes, this kit will isolate small/micro RNA’s ≥ 17 nucleotides.

    Yes, freeze samples at -80°C after samples are lysed/homogenized in DNA/RNA Lysis Buffer. Bring the sample to room temperature prior to RNA purification.

    Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add 1 volume of DNA/RNA Lysis Buffer (1:1) and mix well. Proceed with DNA Purification.

    Cells - Pellet by centrifugation at up to 5,000 x g and remove RNAlater™ (supernatant). Proceed to Sample Preparation, see protocol.

    Tissue - Transfer into a new tube with forceps and remove any excess RNAlater™. Proceed to Sample Preparation, see protocol.

    Alternatively, for liquid samples from which RNAlater™ cannot be removed, add 1 volume of nuclease-free water (or PBS) to 1 volume liquid sample (1:1) and mix. Then add 4 volumes DNA/RNA Lysis Buffer to 1 volume sample/water (or PBS) mixture (4:1). Mix again and proceed to Total RNA Purification, see protocol.

    This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.

    Cat # Name Size
    R1200-25 DNA/RNA Shield (2X Concentrate) 25 ml
    W1001-10 DNase/RNase-Free Water 10 ml
    C1004-50 Zymo-Spin IC Columns 50 Pack
    R1200-1-5 PK Digestion Buffer 5 ml
    C1001-50 Collection Tubes 50 Pack
    D7010-2-50 DNA/RNA Prep Buffer 50 ml
    D7001-1-50 DNA/RNA Lysis Buffer 50 ml
    D7010-3-24 DNA/RNA Wash Buffer (Concentrate) 24 ml
    E1010-1-4 DNA Digestion Buffer 4 mL
    C1103-50 Zymo-Spin IC-XM Columns 50 Pack

    “This kit was invaluable. I was able to get at least 1 ug of RNA and several hundred nanograms of DNA from each sample - all of it high quality. Much better than my previous experience with a different dual DNA/RNA isolation kit.”

    - Julie P. (CU Anschutz Medical Campus)

    “I usually recommend this kit for those who want to extract both DNA and RNA from a single sample. We have even had great quality and yield for single insect samples.”

    - Robert B. (Harvard University)

    "The kit is very economical for simultaneous extraction of high quality DNA and RNA from very small amount of biological samples. More particularly, RNA is of good quality that suits best for applications like next-generation sequencing (RNA-Seq)."

    - Muhammad A. (Northwestern University)

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