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    Quick-DNA/RNA Pathogen MagBead

    R2145 / R2146


    Quick-DNA/RNA Pathogen MagBead

    Cat # Name Size
    R2145 Quick-DNA/RNA Pathogen MagBead 1 x 96 Preps
    R2146 Quick-DNA/RNA Pathogen MagBead 4 x 96 Preps

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    Highlights

    • High-throughput, magnetic-bead based purification of pathogen (virus, bacteria, protozoa) DNA/RNA from a wide variety of vectors (mosquitoes, fleas, ticks, etc.) and tissue types (mammals, birds, etc.).
    • High-quality DNA/RNA is ready for Next-Gen sequencing, RT/qPCR, hybridization, etc.
    • DNA/RNA Shield included for sample collection, inactivation and storage.
    Description

    Quick-DNA/RNA Pathogen MagBead kit is designed for high-throughput purification of pathogen (virus, bacteria, protozoa) DNA and RNA from a wide variety of vectors (mosquitoes, fleas, ticks, etc.) and tissue types (mammals, birds, etc.) collected, transported and stored in DNA/RNA Shield™. DNA/RNA Shield is used for nucleic acid preservation and inactivation of pathogens.

    The kit features a storage/lysis buffer system and can be combined with high density ZR BashingBead™ Lysis Tubes (*recommended) to facilitate complete homogenization of hard-to-lyse samples for efficient nucleic acid isolation. Small (>50 nt) and large (> 200 kb) DNA and RNA are bound to magnetic beads, washed and then eluted.

    The isolated high-quality nucleic acids are suitable for all downstream applications such as Next-Gen sequencing, hybridization-based and RT/qPCR detection.

    For automation scripts and support, email automation@zymoresearch.com


    Binding Capacity 10 µg DNA/RNA per 20 µl ZymoBIOMICS MagBinding Beads.
    Elution Volume ≤ 50 µl ZymoBIOMICS DNase/RNase-Free Water
    Equipment Needed Magnetic stand or separator, liquid handler or robotic sample processer (user provided).
    Materials (avaliable separately):
    ZR BashingBead Lysis Tubes (S6014; 0.1/2.0 mm)
    ZR-96 MagStand (P1005)
    Collection Plate (C2002; capacity 1.2 ml/well)
    96-Well Block (P1001; capacity 2 ml/well)
    Elution Plate (C2003; capacity 0.35 ml/well)
    Cover Foil (C2007; 2, 6, 12 or 24 pack)
    Purity High-quality DNA/RNA ready for Next-Gen sequencing, RT/qPCR, hybridization, etc.
    Sample Type ≤ 10 mg vectors (mosquitoes, fleas, ticks, other tough-to-lyse insects) and tissue types (animal tissue, plants, other hosts) or ≤ 200 µl biological liquids.
    Size 50 nt to ~200 kb
    Yield 10 µg DNA/RNA binding capacity (per 20 µl MagBinding Beads), ≥50 µl elution volume

    This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.




    Cat # Name Size
    R1100-250 DNA/RNA Shield 250 ml
    D4302-5-30 ZymoBIOMICS DNase/RNase Free Water 30 ml
    D4302-5-50 ZymoBIOMICS DNase/RNase Free Water 50 ml
    D4302-6-12 ZymoBIOMICS MagBinding Beads 12 ml
    D4302-6-6 ZymoBIOMICS MagBinding Beads 6 ml
    R1042-1-100 Pathogen DNA/RNA Buffer 100 ml
    R2130-1-120 MagBead DNA/RNA Wash 1 120 ml
    R2130-1-30 MagBead DNA/RNA Wash 1 30 ml
    R2130-2-20 MagBead DNA/RNA Wash 2 20 ml
    R2130-2-80 MagBead DNA/RNA Wash 2 80 ml