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Quick-DNA/RNA Pathogen MagBead
R2145 / R2146
Quick-DNA/RNA Pathogen MagBead
- High-throughput, magnetic-bead based purification of pathogen (virus, bacteria, protozoa) DNA/RNA from a wide variety of vectors (mosquitoes, fleas, ticks, etc.) and tissue types (mammals, birds, etc.).
- High-quality DNA/RNA is ready for Next-Gen sequencing, RT/qPCR, hybridization, etc.
- DNA/RNA Shield included for sample collection, inactivation and storage.
Quick-DNA/RNA Pathogen MagBead kit is designed for high-throughput purification of pathogen (virus, bacteria, protozoa) DNA and RNA from a wide variety of vectors (mosquitoes, fleas, ticks, etc.) and tissue types (mammals, birds, etc.) collected, transported and stored in DNA/RNA Shield™. DNA/RNA Shield is used for nucleic acid preservation and inactivation of pathogens.
The kit features a storage/lysis buffer system and can be combined with high density ZR BashingBead™ Lysis Tubes (*recommended) to facilitate complete homogenization of hard-to-lyse samples for efficient nucleic acid isolation. Small (>50 nt) and large (> 200 kb) DNA and RNA are bound to magnetic beads, washed and then eluted.
The isolated high-quality nucleic acids are suitable for all downstream applications such as Next-Gen sequencing, hybridization-based and RT/qPCR detection.
For automation scripts and support, email firstname.lastname@example.org
|Binding Capacity||10 µg DNA/RNA per 20 µl ZymoBIOMICS MagBinding Beads.|
|Elution Volume||≤ 50 µl ZymoBIOMICS DNase/RNase-Free Water|
|Equipment Needed||Magnetic stand or separator, liquid handler or robotic sample processer (user provided).|
ZR BashingBead Lysis Tubes (S6014; 0.1/2.0 mm)
ZR-96 MagStand (P1005)
Collection Plate (C2002; capacity 1.2 ml/well)
96-Well Block (P1001; capacity 2 ml/well)
Elution Plate (C2003; capacity 0.35 ml/well)
Cover Foil (C2007; 2, 6, 12 or 24 pack)
|Purity||High-quality DNA/RNA ready for Next-Gen sequencing, RT/qPCR, hybridization, etc.|
|Sample Type||≤ 10 mg vectors (mosquitoes, fleas, ticks, other tough-to-lyse insects) and tissue types (animal tissue, plants, other hosts) or ≤ 200 µl biological liquids.|
|Size||50 nt to ~200 kb|
|Yield||10 µg DNA/RNA binding capacity (per 20 µl MagBinding Beads), ≥50 µl elution volume|
|R1100-250||DNA/RNA Shield||250 ml|
|D4302-5-30||ZymoBIOMICS DNase/RNase Free Water||30 ml|
|D4302-5-50||ZymoBIOMICS DNase/RNase Free Water||50 ml|
|D4302-6-12||ZymoBIOMICS MagBinding Beads||12 ml|
|D4302-6-6||ZymoBIOMICS MagBinding Beads||6 ml|
|R1042-1-100||Pathogen DNA/RNA Buffer||100 ml|
|R2130-1-120||MagBead DNA/RNA Wash 1||120 ml|
|R2130-1-30||MagBead DNA/RNA Wash 1||30 ml|
|R2130-2-20||MagBead DNA/RNA Wash 2||20 ml|
|R2130-2-80||MagBead DNA/RNA Wash 2||80 ml|