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    Quick-RNA Viral Kit


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    Cat # Name Size
    R1034 Quick-RNA Viral Kit 50 preps
    R1035 Quick-RNA Viral Kit 200 preps
    R1034-E Quick-RNA Viral Kit – DX 50 preps
    R1035-E Quick-RNA Viral Kit - DX 200 preps
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    Highlights

    • Viral RNA: Compatible with plasma, serum, urine, cell culture media, blood, saliva, cellular suspensions, biopsies, swabs, feces, samples in DNA/RNA Shield, etc.
    • Streamlined Workflow: Sample inactivation and easy one-step lysis enables fast processing.
    • High Sensitivity: Optimized for low viral copy detection for Next-Gen Sequencing and RT-qPCR.
    Description & Documents Specifications FAQ Components Citations

    Documents

    Product Description

    The Quick-RNA Viral Kit is a quick, purification system for viral RNA from plasma, serum, cell culture media, cellular suspensions, urine, blood, saliva and any other biological samples stored in DNA/RNA Shield™. DNA/RNA Shield™ ensures nucleic acid stability during sample storage/transport at ambient temperatures (4-25°C). The reagent effectively lyses cells and inactivates nucleases and infectious agents (virus). The kit also features a specialized buffer system that facilitates complete viral particle lysis for efficient RNA isolation from samples containing enteroviruses, rhinoviruses, coronaviruses, HIV, HCV, influenza A virus, flaviviruses, measles virus, parainfluenza virus, parvovirus (a ssDNA virus), etc. Viral RNA is bound to the column, washed and eluted. The isolated high-quality viral RNA is ready for all downstream applications such as Next-Gen Sequencing, hybridization-based and RT/PCR detection.

    Documents

    Technical Specifications

    Equipment Microcentrifuge
    Purity RNA is ready for Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc.
    Registration Status CE-IVD certified (R1034-E, R1035-E)
    Sample Source Plasma, serum, saliva, urine, blood, cell culture media, cellular suspensions, biopsies, and swab and fecal samples stored in DNA/RNA Shield
    Yield 10 µg RNA (binding capacity), ≥6 µl (elution volume)

    Resources

    The purpose of beta-mercaptoethanol is to help with deproteination. It is not necessary if you are working with simple samples such as swabs. It is recommended if you are working with protein rich samples such as plasma, serum, blood, saliva, sputum, etc.

    Yes, these samples are compatible.

    Yes, this kit will co-purify some DNA.

    Most downstream application methods for viral detection do not require DNase treatment during purification. If necessary, DNase treatment can be perform post-purification and additional components can be purchased separately (i.e., DNase I, DNA Digestion Buffer, RNA Prep Buffer and RNA Wash Buffer).

    Yes, the change from clear to yellow is a result of oxidation and will not affect the performance of the buffer.

    The Viral RNA Buffer is guaranteed for one year from the date of purchase even with the addition of beta-mercaptoethanol. Just be sure to close the bottle cap tightly to prevent evaporation.

    In most cases, it could be because the samples contain high amounts of protein or cellular debris. To help prevent this in future preps, it’s best to add beta-mercaptoethanol to the Viral RNA buffer, and/or implement a Proteinase K digestion step.

    For optimal results and detection of viral target, collect sample in DNA/RNA Shield and perform Proteinase K treatment. In addition, add beta-mercaptoethanol to the Viral RNA Buffer prior to purification, as well as perform all steps at room temperature and centrifugation speeds at 10,000-16,000 x g to ensure no buffer retention.

    The “-E" markings at the end of the catalog numbers of “-Dx” products indicate that they are CE-IVD verified for in vitro diagnostic use.

    This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.

    Cat # Name Size
    R1200-25 DNA/RNA Shield (2X Concentrate) 25 ml
    R1200-125 DNA/RNA Shield (2X Concentrate) 125 ml
    W1001-10 DNase/RNase-Free Water 10 ml
    W1001-4 DNase/RNase-Free Water 4 ml
    R1034-1-100 Viral RNA Buffer 100 ml
    R1034-1-50 Viral RNA Buffer 50 ml
    R1034-2-48 Viral Wash Buffer (concentrate) 48 ml
    R1034-2-6 Viral Wash Buffer (concentrate) 6 ml
    C1001-50 Collection Tubes 50 Pack
    C1004-50 Zymo-Spin IC Columns 50 Pack

    “The Quick-RNA Viral RNA kit performed much better than the RNeasy 96 kit for extracting RNA from dilute samples of virus supernatant. The kit is designed for small-volume elution to automatically concentrate the sample, and it’s designed for viral supernatants rather than cell lysates, so we didn’t need to deal with optimizing added carrier RNA. So in all, it was simpler, easier, and gave us a 10-fold improvement in our limit of detection.”

    - S.B., Boehringer Ingelheim, Canada

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