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    ZymoBIOMICS Oral Microbiome Standard


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    Cat # Name Size
    D6332 ZymoBIOMICS Oral Microbiome Standard 10 preps

    Highlights

    • Accurate representation: Comprised of 12 different strains to mimic the human oral microbiome.
    • Precise composition: allows for benchmarking and validation of NGS microbiome workflows.
    • Assess bias in DNA isolation: contains both Gram-positive and negative bacteria sorted in a staggered abundance to assess profiling bias and detection limits.
    Description & Documents Specifications FAQ Related Products

    Documents


    Product Description


    The ZymoBIOMICS Oral Microbiome Standard is a an accurately quantified microbial community mimicking the human oral microbiome. The challenges it presents to microbiomics profiling allows researchers to validate protocols and fine-tune them for oral microbiome research and act as a positive control for sample processing.

    Documents

    Technical Specifications

    Biosafety This product contains no biohazard as microbes have been fully inactivated.
    Impurity level < 0.01% foreign microbial DNA
    Reference genomes and 16S&18S rRNA genes https://s3.amazonaws.com/zymo-files/BioPool/D6332.refseq.zip
    Relative-abundance deviation in average <30%
    Storage solution DNA/RNA Shield™ (Cat. No. R1100)
    Total cell concentration ~1.3 x 109 cells/ml

    Resources


    For users that are setting up a new microbiomics workflow, we recommend starting with the D6300 Microbial Community Standard. The lower complexity of the standard will enable the user to more easily detect existing biases in the workflow (i.e. lysis during DNA extraction). Users who have validated using the D6300 already may then use the D6332 to fine-tune their workflows for oral microbiome applications.

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