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    Zymolyase Ultra


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    Cat # Name Size
    E1007T Zymolyase Ultra 100 U
    E1007-2 Zymolyase Ultra 2,000 U
    E1007-10 Zymolyase Ultra 10,000 U
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    Highlights

    • Ultra Efficient: 50x more efficient than lyticase.
    • Ultra Low-Bioburden: Up to 70x less DNA contamination compared to other suppliers.
    • Convenient to Use: Dissolves in water and stable for multiple freeze-thaw cycles.
    Description & Documents Application Specifications FAQ

    Documents

    Product Description

    Digestion of yeast and fungal cell walls is necessary for many experimental procedures. Lytic enzymes like Zymolyase, Lyticase, Glucanase or Chitinase are routinely used for digestion of cell walls. Distinguished from conventional lytic enzymes, Zymolyase Ultra is a novel formulation of enzyme that is optimized for efficient yeast cell wall digestion. It contains enzymes that can degrade several different components of yeast cell walls. It is extensively purified using a novel DNA/RNA removal technology that results in extremely low nucleic acid contamination. It is suitable for qPCR and sequencing-based assays where low DNA/RNA contamination is critical.

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    Documents

    Application

    • Plasmid, DNA and RNA purification: By breaking down the rigid cell walls of yeast and fungi, Zymolyase Ultra facilitates the release of nucleic acids and significantly increases nucleic acid extraction efficiency.
    • Protein purification: Zymolyase Ultra enables efficient extraction of proteins from the cell wall, cell membrane, and intracellular compartments. Unlike mechanical lysis, the gentle enzymatic lysis protects sensitive proteins from denaturation caused by heating or physical shearing. It supports downstream analyses such as protein-protein interaction assays and antibody-based assays, Western blotting, mass spectrometry, and proteomic studies.
    • Yeast/fungi detection: Increase cell lysis facilitated by Zymolyase Ultra can directly contribute to enhanced sensitivity of yeast/fungi detection, critical for sensitive analysis like PCR, qPCR and NGS.
    • Protoplast/Spheroplast preparation: With gentle enzymatic breakdown of yeast cell walls, cellular structures and components are protected from mechanical stress and damage.
    • Yeast genetics and cell biology: Lysing yeast cells using Zymolyase Ultra with ultra-low DNA contamination ensures the extraction of clean and uncontaminated genetic material, minimizing the risk of introducing foreign DNA.

    Technical Specifications

    Activity β-1,3-glucan laminaripentao-hydrolase
    β-1,3-glucanase
    β-1,6 glucanase
    Chitinase
    Trace amount of protease
    Format Lyophilized
    Optimum pH 7.3 - 8.0
    Optimum Temperature 37°C
    Reconstitution Add nucleic acid-free and DNase/RNase-free water to the lyophilized Zymolyase Ultra to 1-5 U/µl, mix by gentle inversion.
    Solubility ≤ 5U/µl in water
    Storage After reconstitution store frozen aliquots (-20°C).

    Resources

    Yes, we can customize your order request to meet your specific requirements. For bulk ordering, please click “Bulk Order Inquiry”.

    There are two main differences:
    1. Zymolyase Ultra has lower DNA/RNA contamination.
    2. Zymolyase Ultra consists of more lytic enzymes targeting different cell wall components, thus it has higher cell wall lysing efficiency.

    DNA copies were calculated by using Zymo Research Femto Bacterial DNA Quantification kit (E2006) and Femto Fungal DNA Quantification kit (E2007). These are qPCR-based assays that either amplify the 16S or ITS regions and compare an unknown sample against a standard curve of known DNA amounts. The measurements were converted to genomic DNA copies/Unit enzyme in each assay and then combined for total DNA content.

    It is active between 4-37°C.

    It is active in common buffers like Tris-Cl and phosphate buffers. Optimal pH is between 7.3-8.0.

    Zymolyase Ultra is very efficient, but here are some tips that can improve cell wall digestion efficiency:
    1. Use fresh and early log phase cells, which are more susceptible to enzymatic lysis.
    2. Using less cells as input can improve overall digestion efficiency.
    3. Increase digestion time.
    4. Add 1-2 mM β-mercaptoethanol to the reaction.

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