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    Zyppy-96 Plasmid MagBead Miniprep


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    Cat # Name Size
    D4100 Zyppy-96 Plasmid MagBead Miniprep 2 x 96 Preps.
    D4101 Zyppy-96 Plasmid MagBead Miniprep 4 x 96 Preps.
    D4102 Zyppy-96 Plasmid MagBead Miniprep 8 x 96 Preps.

    Highlights

    • Pellet-Free: Rapid direct lysis procedure omits cell-pelleting and resuspension steps
    • High Quality: Ready for PCR, sequencing, cloning, and transfection
    • Ideal for Synthetic Biology: Fastest, high-throughput automated method for preparing high-quality plasmid DNA.
    Description & Documents Specifications FAQ Components

    Documents

    Supplemental Info

    Product Description

    The Zyppy-96 Plasmid MagBead Miniprep is the fastest high-throughput (96-well), Pellet-Free plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are expedited using a specially designed Neutralization Buffer. Plasmid DNA is then purified using the featured magnetic bead based technology. This straightforward procedure is compatible for either automated or manual processing. Eluted plasmid DNA is of the highest quality, endotoxin-free, and is well suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection. An overview of the purification procedure is shown below.

    Documents

    Technical Specifications

    Applicable For Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.
    Elution Volume ≥ 30 µl
    Equipment Magnetic Stand, Heating Element, or Liquid Handler
    Processing Time 60 min
    Purity Typical Abs 260/280 ≥1.8.
    Size Range Up to 25 kb
    Yield Up to 5 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli processed.
    Supplemental Info

    Resources

    10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.

    Yes, all our plasmid preps have an endo-wash step to remove endotoxin very effectively and produce transfection ready high-quality plasmid DNA.

    No, we recommend using our ZR BAC DNA Miniprep Kit or ZymoPURE II Kits for isolating large constructs.

    ≤ 50 Endotoxin Units/µg of plasmid DNA.

    Zyppy Wash Buffer is available for purchase, separately. However, you can substitute with DNA Wash Buffer, Plasmid Wash Buffer, or 80% ethanol.

    Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4 °C as soon as possible to ensure optimal performance throughout the life span of the product.

    Cat # Name Size
    C2003 Elution Plate 2 Plates
    C2002 Collection Plate 2 Plates
    P1001-2 96-Well Block 2 Blocks
    C2007-12 96-Well Plate Cover Foil 12 Foils
    C2011-2 Air Permeable Sealing Cover 2 Pack
    C2011-4 Air Permeable Sealing Cover 4 Pack
    C2011-8 Air Permeable Sealing Cover 8 Pack
    C2007-6 96-Well Plate Cover Foil 6 Foils
    C2007-24 96-Well Plate Cover Foil 24 Foils
    D4036-3-120 Endo-Wash Buffer 120 ml
    D4036-2-100 Neutralization Buffer (Yellow) 100 ml
    D4036-2-200 Neutralization Buffer (Yellow) 200 ml
    D4036-3-240 Endo-Wash Buffer 240 ml
    D4036-3-60 Endo-Wash Buffer 60 ml
    D4036-4-48 Zyppy Wash Buffer (Concentrate) 48 ml
    D4036-5-100 Zyppy Elution Buffer 100 ml
    D4036-5-30 Zyppy Elution Buffer 30 ml
    D4041-1-48 Deep Blue Lysis Buffer 48 ml
    D4041-1-30 Deep Blue Lysis Buffer 30 ml
    D4036-5-60 Zyppy Elution Buffer 60 ml
    D4100-2-8 MagBinding Beads 8 ml
    D4100-1-10 MagClearing Beads 10 ml
    D4100-1-20 MagClearing Beads 20 ml
    D4100-1-40 MagClearing Beads 40 ml
    D4100-2-16 MagBinding Beads 16 ml

    “The speed I was doing a side by side comparison with Invitrogen and it is really nice to go from culture to plasmid DNA in less than 15 min.  Also the precipitate was really solid, no floaties, so it was very easy to pipet off the supernatant.”

    - Rebecca N.

    “It performs just as well as the Qiagen mini-prep kit (the accepted lab-standard).  The kit was very easy to follow and the resultant DNA was compatible with our complex downstream procedures.”

    - Richard F.

    "The results gave what they promised!  Clean DNA.">/em<

    - Pamela R (UT Medicine San Antonio)

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