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16S/ITS Amplicon Sequencing Service
Identification of Bacteria, Archaea, & Fungi From Any Microbiome Sample
Quick Turnaround Time
Receive 16S/ITS amplicon extraction and sequencing results in as little as 1 week
Unparalleled Data Resolution
Species-level identification with 16S rRNA sequencing service
Absolute Abundance Quantification
Reveal differences that would be missed with relative abundance alone
Comprehensive Bioinformatics Analysis
Simplify 16S/ITS Amplicon Sequencing Data Interpretation
All projects include a comprehensive report with bioinformatics analysis and raw sequencing data. The 16S rRNA sequencing service report provides compositional bar plots, taxonomy heatmaps, alpha-diversity and beta diversity analyses. Group comparisons and LEfSe biomarker discovery are also reflected in all 16S/ITS amplicon sequencing service reports. Our team of expert bioinformaticians are here to help you get the most out of your data.
Achieve Species-Level Identification With 16S Amplicon Sequencing
Zymo Research 16S Pipeline
Other Service Providers
By combining leading edge sequencing error correction methods with a highly curated 16S rRNA database, our 16S amplicon sequencing service can provide taxonomic identification down to the species level.
Gain Unique Insights Into Your Data
Zymo Research's Absolute Abundance Quantification Reveals More
Relative abundance alone cannot define changes in a microbial community. In cases like comparing treatment or
therapeutic effects, it can be just as important to know how cell numbers are changing. With absolute abundance
quantification provided by our 16S amplicon sequencing service, open new insights into the microbial community dynamics of your
Rigorously Optimized For The Highest Data Accuracy
Our 16S amplicon sequencing service is continually monitored with mock community controls to ensure data accuracy and
consistency. Don’t let run-to-run variations interfere with your long-term study or project.
16S/ITS Amplicon Sequencing Made Simple
How Our Straightforward, User-Friendly Service Works
Blocks amplification of the V4 region of chloroplast and mitochondrial rRNA genes
Quantification of absolute 16S or ITS gene copy number using qPCR with library preparation primers
A positive control for DNA extraction (ZymoBIOMICS Microbial Community Standard, D6300) and a positive control for
library preparation (ZymoBIOMICS Microbial Community DNA Standard, D6305) are included in each project as a quality
control. Results are reported in the final project report. Negative extraction controls and negative qPCR controls are
also monitored with real time PCR and can be analyzed upon request.
Amplicon Sequence Variant (ASV) based analysis with DADA2 is used to provide higher accuracy and better resolution of data.
As publicly available databases (e.g. Greengenes, UNITE) contain a multitude of errors, inconsistent annotations, and poor sequence quality, we utilized an in-house curated reference database.
Absolute abundance boxplot, taxa abundance barplot, taxa abundance heatmap, ASV abundance heatmap, alpha diversity boxplot and rarefaction curves, beta diversity 3D Emperor plot, Taxa2ASV decomposer
LEfSe analysis uncovers taxa/traits that are differentially distributed among defined sample groups.
We have processed a wide variety of sample types, from human tissue samples, environmental samples, to food samples. If
you have questions or concerns, please feel free to fill out an inquiry or contact us.
Because of our included quality control standard, it is possible
to batch samples over time and have confidence that the samples were processed in the same way. We recently completed a
project that had a total of >2,000 samples processed over a period of 6 months. By comparing the results from the
standard for each group of samples, we had confidence that the results from different batches could be compared
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