Low-input RRBS DNA Methylation Profiling
From as Little as 10ng of DNA
Reduced Representation Bisulfite Sequencing (RRBS) Is Informative and Efficient
Needs only ~ 10% of sequencing reads compared to whole-genome bisulfite sequencing
Covers ≥70% of promoters, CpG islands, gene bodies, and ~ 35% of enhancers*
Ideal for high throughput DNA methylation screening at single nucleotide resolution
RRBS Is Applicable Across Diverse Species
RRBS Has Many Popular Applications
Drug Response Study
Zymo-Seq RRBS Provides A Simple Solution for NGS Library Preparation
The workflow minimizes hands-on time.
Zymo-Seq RRBS Detects a Significant Number of CpG Sites
Distribution of CpG sites detected (≥ 5X, green dots) in a library constructed with Zymo-Seq RRBS Library Kit across the human chromosomes using 10 ng of human liver genomic DNA
Zymo-Seq RRBS Delivers Reproducible Results Regardless of Input Amount
CpG Sites Overlap
Methylation Ratio Correlation
Libraries constructed with Zymo-Seq RRBS Library Kit using 10 ng and 100 ng of human liver genomic DNA.
Left: Overlap of CpG sites detected (≥5X). Right: Methylation ratio correlation of CpG sites detected (≥50X)
Simplify Your Research With The Zymo-Seq RRBS Library Kit
* Enhancers derived from H3K4me2 peaks. Data based on libraries generated using Zymo-Seq RRBS Library Kit with various amount of human liver genomic DNA.