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    5-Fluoroorotic Acid (5-FOA)


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    Cat # Name Size
    F9001-1 5-Fluoroorotic Acid (powder) 1 g
    F9001-5 5-Fluoroorotic Acid (powder) 5 g
    F9003 100X 5-Fluoroorotic Acid (Liquid) 10 ml

    Highlights

    • Yeast Genetic Counter-Selection Agent: Commonly used for curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens.
    • Convenient: Available as a pure powder or ready-to-use solution in DMSO.
    • Ultra-Pure: Determined > 98% by thin-layer chromatography (TLC), melting point, and lot comparison.
    Description & Documents Specifications

    Documents

    Product Description

    Using 5-Fluoroorotic Acid (5-FOA) for the counter-selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. Otherwise nontoxic to yeast, 5-FOA is converted to the toxic form (i.e., 5-flurouracil) in strains expressing the functional URA3 gene coding for orotine-5-monophosphate decarboxylase that is involved in the synthesis of uracil. Yeast strains that are phenotypically Ura+ become Ura- and 5-FOA(R) (resistant) after selection. The question of 5-FOA solubility is often raised by customers using ultra-pure (< 98%) 5-FOA powder because of its insolubility in water. Thus, we provide a 100X concentrated (100 mg/ml) 5-FOA solution in DMSO and synthetic 5-FOA monohydrate powder. Both have been tested and validated on the basis of counter selection activity.

    Documents

    Technical Specifications

    Format Synthetic 5-FOA monohydrate powder or 100X liquid (100 mg/ml in DMSO)
    Molecular Weight 174 g/mol
    Product Storage Store at -20°C
    Purity ≥ 98% (estimated) by TLC, melting point, and lot comparison
    Solubility 50 mg in 1 ml (1:1 1:1 NH4 OH:H2O) with gentle heating; >100 mg/ml in DMSO
    Structure C5H3FN2O4• H2O

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