ChIP DNA Clean & Concentrator

D5205 / D5201


ChIP DNA Clean & Concentrator

Cat # Name Size
D5205 ChIP DNA Clean & Concentrator (Capped Columns) 50 Preps
D5201 ChIP DNA Clean & Concentrator (Uncapped Columns) 50 Preps
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Highlights
  • Two minute DNA clean-up from any step in a standard ChIP protocol.
  • DNA is ideal for PCR, arrays, DNA quantification, Southern blot analysis, sequencing, and other molecular applications.
Description

The Chromatin Immunoprecipitation (ChIP) DNA Clean & Concentrator provides a hassle-free method for the rapid purification and concentration of high-quality DNA from any step in a standard ChIP protocol. This includes samples that have undergone reverse cross-linking, Proteinase K or RNase A digestion, mechanical or nuclease-mediated DNA shearing, and samples eluted from chromatin-antibody-bead complexes. The specially formulated ChIP DNA Binding Buffer promotes DNA adsorption to the column in the presence of detergents, antibodies, and proteinases that are often used for ChIP.

Elution Volume ≥ 6 µl
Processing Time 2 minutes
Recovery 70-90% for DNA 50 bp - 10 kb
70% for DNA>10 kb
Size Range 50 bp - 23 kb
Storage All components should be stored at room temperature.
Cat # Name Size
D3004-4-10 DNA Elution Buffer 10 ml
D4003-2-6 DNA Wash Buffer (Concentrate) 6 ml
C1004-50 Zymo-Spin IC Columns 50 Pack
C1003-50 Zymo-Spin I Columns 50 Pack
C1001-50 Collection Tubes 50 Pack
D5201-1-50 ChIP DNA Binding Buffer 50 ml