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    DNA Clean & Concentrator-25

    D4033 / D4034 / D4005 / D4006


    DNA Clean & Concentrator-25

    Cat # Name Size
    D4033 DNA Clean & Concentrator-25 (Capped) 50 Preps
    D4034 DNA Clean & Concentrator-25 (Capped) 200 Preps
    D4005 DNA Clean & Concentrator-25 (Uncapped) 50 Preps
    D4006 DNA Clean & Concentrator-25 (Uncapped) 200 Preps

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    Highlights

    • Quick (2 minute) desalting and recovery of ultra-pure DNA from enzymatic reactions (e.g., PCR and endonuclease digestions), cell-free lysates, etc.
    • Column design allows DNA to be eluted at high concentrations into minimal volumes.
    • Eluted DNA is well-suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
    Description

    The DNA Clean & Concentrator-25 (DCC-25) is a PCR purification kit designed for rapid desalting and purification of up to 25 µg DNA from enzymatic reactions (e.g., PCR), endonuclease digestions, or cell-free lysates. Simply add the specially formulated DNA Binding Buffer to your sample and transfer to the supplied Zymo-Spin column. This PCR purification kit features Zymo-Spin column technology to yield high-quality, purified DNA in just minutes, and it is compatible with cDNA and ssDNA. Eluted DNA is suitable for sequencing, microarray analysis, PCR, nucleotide blotting, and restriction endonuclease digestion procedures.


    Detergent Tolerance ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS
    Elution Volume ≥ 25 µl
    Equipment Microcentrifuge
    Purity Highly-pure DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions.
    Sample Source DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.
    Size Range 50 bp to 23 kb
    Yield ≤ 25 µg total DNA can be recovered. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.

    The only difference is the cap. Everything else between the columns (max capacity, column matrix, etc.) is the same. Unsure which to use? It mainly comes down to preference. Some people like the capped columns for easy labeling or added security while others like the uncapped columns for faster sample processing.

    Working with volumes below 50 µl can result in decreased recovery. Zymo Research recommends raising the starting volume to 100 µl with water to ensure optimal binding conditions.

    Zymo Research recommends reloading columns no more than 5 times, as binding efficiency might decrease.

    Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.

    Add an equal volume of ethanol (95-100%) to the sample and mix well. The sample is ready-to-bind and does not require DNA Binding Buffer. Proceed to Step 2.

    Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.

    The DNA will be eluted off the column during the wash step. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.

    We have a Zymoclean Gel DNA Recovery Kit that has chemistry compatible with agarose gel. However, if you have the DNA Clean & Concentrator kits on hand, you can clean up DNA from gel. You would need to purchase the Agarose Dissolving Buffer (Cat# D4001-1-50, D4001-1-100) separately, and follow the Zymoclean Gel DNA Recovery Kit protocol (link here).




    “This kit performed as described. I was able to obtain good quality and yield of the DNA -better than with a couple of competitor's kits.”

    - Kathleen B. (SUNY-ESF)

    “The simple steps and washing instructions were excellent. DNA was ready for use for PCR and gave strong results.”

    - Tyler C.

    “It only took 2 minutes for the total procedure - shorter than my current method of PCR purification.”

    - Marissa V. (Harvard Medical School)

    Read More

    Cat # Name Size
    C1078-50 Zymo-Spin IICR Columns 50 Pack
    C1078-250 Zymo-Spin IICR Columns 5 x 50 Pack
    D4003-2-6 DNA Wash Buffer (Concentrate) 6 ml
    D4004-1-L DNA Binding Buffer 100 ml
    D4003-1-L DNA Binding Buffer 50 ml
    D4003-2-24 DNA Wash Buffer (Concentrate) 24 ml
    D3004-4-10 DNA Elution Buffer 10 ml
    D3004-4-4 DNA Elution Buffer 4 ml
    C1001-50 Collection Tubes 50 Pack
    C1008-50 Zymo-Spin II Columns 50 Pack
    C1008-250 Zymo-Spin II Columns 5 x 50 Pack
    C1001-500 Collection Tubes 500 Pack
    C1001-1000 Collection Tubes 1000 Pack