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    DNase I Set (RNase-free)


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    Read The Zymo Research Promise

    Cat # Name Size
    E1010 DNase I Set 250 U
    E1011 DNase I Set 1500 U
    E1012 DNase I Set 5 x 1500 U
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    Highlights

    • Highest Activity: Complete and efficient DNA digestion for sensitive downstream applications like RNA sequencing.
    • RNase-free and Protease-free: Rigorously tested and guaranteed to be free of contaminating RNases and proteases.
    • Exceptional Stability: Maintains activity after vigorous vortexing and multiple freeze-thaw cycles.
    Description & Documents Specifications Components

    Documents

    Product Description

    Zymo Research DNase I is a recombinant endonuclease that cleaves single- and double-stranded DNA. It digests DNA more efficiently than other DNase I, making it optimal for sensitive applications like NGS (RNA-seq) and PCR. It is extensively purified, and quality controlled to ensure RNase-free and protease-free.

    Zymo Research DNase I is lyophilized and supplied with DNA digestion buffer. It dissolves in water within seconds and remains stable for up to 10 freeze-thaw cycles. Customized, OEM, and bulk orders are available.

    Documents

    Technical Specifications

    1Unit Definition One Kunitz causes an increase in absorbance at 260 nm of 0.001 per minute per ml, at 25°C, pH 7.5, when acting on salmon sperm DNA according to the assay method of Kunitz. One Unit of DNase I is equivalent to 12 Kunitz.
    2Storage After reconstitution, place on ice until ready to use or store frozen aliquots (-20°C).
    3Reconstitution Add DNase/RNase-free water to the lyophilized DNase I, mix by gentle inversion. Avoid phosphate buffer and calcium chelators.
    4Inactivation Heat inactivate at 75 °C for 10 min with 5 mM EDTA.

    Resources

    • Remove contaminating DNA from RNA or protein samples
    • Clean up of RNA samples prior to RT-PCR
    • DNA-free RNA sample preparation prior to RNA-seq
    • Remove DNA templates after in vitro transcription
    • DNA labeling by nick-translation
    • Reduce viscosity in cell culture or cell lysate
    • DNase I footprinting
    Cat # Name Size
    E1010-1-4 DNA Digestion Buffer 4 mL
    E1010-1-16 DNA Digestion Buffer 16 mL

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