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    DNase I Set (RNase-free)

    Zymo Research DNase I is recombinant, animal-free and RNase-free. It nonspecifically digests single- and double-stranded DNA in-solution and on-column. Explore now.

    Highlights

    • Highest Activity: Complete and efficient DNA digestion for sensitive downstream applications like RNA sequencing.
    • RNase-free and Protease-free: Rigorously tested and guaranteed to be free of contaminating RNases and proteases.
    • Exceptional Stability: Maintains activity after vigorous vortexing and multiple freeze-thaw cycles.
    Zymo Research's 100% Satisfaction Guarantee

    Original Manufacturer

    Innovated in California, Made in the USA

    Satisfaction 100% guaranteed, read Our Promise

    Highlights

    • Highest Activity: Complete and efficient DNA digestion for sensitive downstream applications like RNA sequencing.
    • RNase-free and Protease-free: Rigorously tested and guaranteed to be free of contaminating RNases and proteases.
    • Exceptional Stability: Maintains activity after vigorous vortexing and multiple freeze-thaw cycles.
    Zymo Research's 100% Satisfaction Guarantee

    Original Manufacturer

    Innovated in California, Made in the USA

    Satisfaction 100% guaranteed, read Our Promise

    Cat # Name Size
    E1010 DNase I Set 250 U
    E1011 DNase I Set 1500 U
    E1012 DNase I Set 5 x 1500 U
    Limited time offer!

    Bulk Order Inquiry

    Cat # Name Size
    E1010-1-4 DNA Digestion Buffer 4 mL
    E1010-1-16 DNA Digestion Buffer 16 mL

    Description

    Zymo Research DNase I is a recombinant endonuclease that cleaves single- and double-stranded DNA. It digests DNA more efficiently than other DNase I, making it optimal for sensitive applications like NGS (RNA-seq) and PCR. It is extensively purified, and quality controlled to ensure RNase-free and protease-free.

    Zymo Research DNase I is lyophilized and supplied with DNA digestion buffer. It dissolves in water within seconds and remains stable for up to 10 freeze-thaw cycles. Customized, OEM, and bulk orders are available.

    Performance

    Technical Specifications
    1Unit Definition One Kunitz causes an increase in absorbance at 260 nm of 0.001 per minute per ml, at 25°C, pH 7.5, when acting on salmon sperm DNA according to the assay method of Kunitz. One Unit of DNase I is equivalent to 12 Kunitz.
    2Storage After reconstitution, place on ice until ready to use or store frozen aliquots (-20°C).
    3Reconstitution Add DNase/RNase-free water to the lyophilized DNase I, mix by gentle inversion. Avoid phosphate buffer and calcium chelators.
    4Inactivation Heat inactivate at 75 °C for 10 min with 5 mM EDTA.

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