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Read The Zymo Research PromiseE. coli Non-Methylated Genomic DNA
| Cat # | Name | Size |
|---|
Highlights
- Negative control for epigenetic experiments requiring DNA containing zero background levels of cytosine and adenosine methylation. Substrate for DNA methylation-sensitive restriction enzyme digestions.
Documents
Supplemental Info
Product Description
Technical Specifications
| Concentration | 250 ng/µl in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) |
|---|---|
| Genotype | ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2 |
| Storage | ≤ -20°C |
Resources
Q1: How do you use these standards?
During NGS library prep, this standard can be spiked in to or run in parallel with experimental samples to monitor bisulfite conversion efficiency and/or workflow performance.
Q2: What reference genome should I use for alignment?
The reference genome of E. coli strain K-12 substrain MG1655 can be used for alignment and analysis. It can be accessed here.
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