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    Genomic DNA Clean & Concentrator-10


    Zymo Research's 100% Satisfaction Guarantee

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    Read The Zymo Research Promise

    Cat # Name Size
    D4011 Genomic DNA Clean & Concentrator-10 100 Preps
    D4010 Genomic DNA Clean & Concentrator-10 25 Preps
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    Highlights

    • Quick, 5-minute clean-up of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations.
    • Unique spin column for low volume elution of ultra-pure, high-yield DNA.
    • Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc.
    Description & Documents Specifications FAQ Components Citations

    Documents

    Product Description

    The Genomic DNA Clean & Concentrator-10 (gDCC) is a gDNA clean up kit that provides quick, 5 minute recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, WGA DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied Zymo-Spin Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. This gDNA clean up kit is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.

    Documents

    Technical Specifications

    Applicable For Eluted DNA is ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, restriction digestion procedures, and any other sensitive downstream application
    Elution Volume ≥ 10 µl of DNA Elution Buffer
    Equipment Microcentrifuge
    Purity A260/A280 > 1.8, A260/A230 > 1.8
    Sample Source Enzymatic reactions or impure preparations containing genomic DNA.
    Sample Storage Eluted DNA can be used immediately or stored at ≤ -20°C.
    Size Range 50 bp to 200 kb
    Yield Recovery of DNA ranges from 70 - 95%

    Resources

    Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.

    Add an equal volume of ethanol (95-100%) to the sample and mix well. The sample is ready-to-bind and does not require DNA Binding Buffer. Proceed to Step 2.

    The DNA will be eluted off the column during the wash step. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.

    Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.

    Zymo Research recommends no more than 5 times, as binding efficiency might decrease.

    Working with volumes below 50 µl can result in decreased recovery. Zymo Research recommends raising the starting volume to 100 µl with water to ensure optimal binding conditions.

    Cat # Name Size
    C1002-25 Zymo-Spin IC-XL 25 Pack
    C1002-50 Zymo-Spin IC-XL 50 Pack
    D5201-1-50 ChIP DNA Binding Buffer 50 ml
    C1001-50 Collection Tubes 50 Pack
    C1001-500 Collection Tubes 500 Pack
    C1001-1000 Collection Tubes 1000 Pack
    D4003-2-6 DNA Wash Buffer (Concentrate) 6 ml
    D4003-2-24 DNA Wash Buffer (Concentrate) 24 ml
    D3004-4-1 DNA Elution Buffer 1 ml
    D3004-4-4 DNA Elution Buffer 4 ml

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