Attention: This product (D6030) is slated for discontinuation on July 1st, 2025. Click here to find the updated version of the kit: OneStep PCR Inhibitor Removal Kit (D6031)

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    OneStep PCR Inhibitor Removal Kit


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    Read The Zymo Research Promise

    Cat # Name Size
    D6035 OneStep-96 PCR Inhibitor Removal Kit 2 x 96 Preps
    D6030 OneStep PCR Inhibitor Removal Kit 50 Preps

    Highlights

    • Removes PCR inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanin, etc. from nucleic acid solutions to yield high quality DNA or RNA.
    • Fast, one-step procedure for cleaning impure samples prior to PCR, sequencing, reverse transcription (RT), etc.
    Description & Documents Specifications FAQ Components Citations

    Documents

    Product Description

    The OneStep PCR Inhibitor Removal Kits are PCR inhibitor clean up kits that contain all the components needed for efficient removal of contaminants that can inhibit downstream enzymatic reactions (e.g. PCR and RT) from DNA and RNA preparations. The column matrices have been specifically designed for the efficient removal of polyphenolic compounds, humic/fulvic acids, tannins, melanin, etc. from the most impure DNA and RNA preparations. Sample clean-up is as simple as applying, spinning, and recovering a sample from the column.

    Documents

    Technical Specifications

    Applicable For Cleaned samples are ready for PCR, sequencing, reverse transcription (RT), etc.
    Elution Volume 50 - 200 µl
    Equipment Microcentrifuge
    Purity High quality, enzymatic-reaction ready DNA/RNA products.
    Sample Source DNA/RNA samples contaminated with enzymatic reaction inhibitors.
    Sample Storage Eluted DNA/RNA can be used immediately or stored at ≤ -20°C.
    Type DNA and RNA
    Yield 50 - 90% recovery

    Resources

    The smallest volume will be 50 ul.

    The OneStep PCR Inhibitor Removal Kit can process about 200 µl of sample at a time. However, the same column can be reused for the same sample.

    No, the kit requires the speed to be at 3,500 x g. Centrifuging at lower speeds can result in inefficient sample elution.

    Cat # Name Size
    C1001-50 Collection Tubes 50 Pack
    D6035-1-30 Prep Solution 30 ml
    C1058-50 Zymo-Spin III-HRC Filters 50 Pack
    C2003 Elution Plate 2 Plates
    C2009 Silicon-A-HRC Plate 2 Plates

    “The ease. Holy cow, this thing was so freaking simple compared to similar products”

    - Christian H. (University of Georgia)

    “It was VERY fast and yielded the intended results...PCR products from previously non-amplifiable DNA.”

    - Tara N. (United States Agricultural Research Service)

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