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Highlights
- Highest Specificity: Selectively digests dsDNA and DNA in DNA-RNA hybrids; virtually inactive towards ssDNA or RNA.
- Premium Purity: RNase-free, protease-free, and animal-free.
- Thermostable: Able to withstand high temperatures without denaturation or loss in activity.
Original Manufacturer
Satisfaction 100% guaranteed, read Our Promise
Innovated in California, Made in the USA
Highlights
- Highest Specificity: Selectively digests dsDNA and DNA in DNA-RNA hybrids; virtually inactive towards ssDNA or RNA.
- Premium Purity: RNase-free, protease-free, and animal-free.
- Thermostable: Able to withstand high temperatures without denaturation or loss in activity.
Original Manufacturer
Satisfaction 100% guaranteed, read Our Promise
Innovated in California, Made in the USA
| Cat # | Name | Size | |
|---|---|---|---|
| E1020-4-50 | 2X DSN Stop Solution | 50 mL | |
| E1020-2-5 | 10X DSN Digestion Buffer | 5 mL | |
Description
Performance
Technical Specifications
| Unit Definition | One Kunitz unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml, at 25°C, pH 7.15, when acting on calf thymus DNA. |
|---|---|
| Concentration | 1 U/µl |
| Optimal Temperature | 60 °C |
| Reaction Buffer | 10X DSN Digestion Buffer 500 mM Tris-HCl, 50 mM MgCl2, pH 8.0 |
| Stop Solution | 2X DSN Stop Solution 10 mM EDTA, pH 8.0 |
| Storage Condition | For long-term storage, store at -20 °C. |
| Inactivation | Add the 2X DSN Stop Solution to the reaction, to a final concentration of 1X, and incubate at the reaction temperature for 5 minutes. |
Resources
Documents
FAQ
- This depends on the application. DSN is a highly versatile enzyme, and the input amount can be adjusted to meet specific requirements. The amount of DSN and incubation times will vary based on the desired product. For example, a typical amount of DSN used for the normalization of cDNA libraries ranges from 0.25 to 1 U.
- To protect ssDNA in a pool of dsDNA/ssDNA, we recommend titrating the enzyme amount to determine the optimal conditions for ssDNA protection.
The incubation temperature can be adjusted between 25°C and 70°C, with an optimal temperature of 60°C.
DSN is susceptible to EDTA inactivation at concentrations of 5 mM and above. We recommend performing an inactivation step by adding 5 mM EDTA at the reaction temperature for 5 minutes following digestion.
DSN is thermally stable and retains activity when heated to 98°C for 10 minutes. We suggest stopping all reactions with DSN by incubating with 5 mM EDTA for 5 minutes at 60°C.
Yes, we can customize your order request to meet your specific requirements. For bulk ordering, please fill out the form and one of our specialists will be in touch shortly.
Applications
- Remove gDNA and DNA in DNA:RNA hybrids for protein or RNA preparations.
- Reduce abundant rRNA and globin transcripts to enhance next-generation sequencing (NGS) libraries.
- Achieve precise single-nucleotide polymorphism (SNP) detection with minimal off-target effects.
- Enhance signal amplification for accurate virus detection.
- Improve detection of miRNA and biomarkers.
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