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    Quick-DNA FFPE Miniprep

    D3067


    Quick-DNA FFPE Miniprep

    Cat # Name Size
    D3067 Quick-DNA FFPE Miniprep 50 Preps

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    Highlights

    • Streamlined purification of high-quality FFPE tissue DNA that is ideal for PCR, Next-Gen library prep, enzymatic manipulations, etc.
    • Size selection technology; recover total DNA >50 bp or >500 bp.
    Description

    The Quick-DNA FFPE Kit provides a simple and reliable method for high yield/quality DNA isolation from formalin-fixed, paraffin embedded (FFPE) tissue samples and sections. The unique chemistries of the product have been optimized for maximum recovery of non-crosslinked, ultra-pure DNA without RNA contamination. Simply digest deparaffinized tissues using the provided Proteinase K, heat, and then purify the DNA with the Zymo-Spin columns in the kit. DNA >50 bp or >500 bp can be selectively isolated by altering the lysis buffer conditions as described in the protocol. PCR inhibitors are effectively removed during the isolation procedure and eluted DNA is ideal for PCR, Next-Gen library prep, enzymatic manipulation, etc.


    Applicable For All sensitive downstream applications such as qPCR and Next-Generation sequencing.
    Equipment Microcentrifuge, heat block/bath (55°C and 90°C), isopropanol, beta-mercaptoethanol (optional).
    Processing Volume Tissue - ≤25 mg or (4) 20 µm thick with a surface area of 20 mm2
    Purity Typical A260/A280 ≥ 1.8
    Sample Source Tissue from paraffin block or tissue sections. Compatible with fresh/frozen tissue specimens
    Sample Storage Eluted DNA should be stored at ≤ -20°C.
    Size Range > 50 bp
    Supplemental Info
    Type Total DNA
    Yield Up to 25 µg total DNA can be eluted into ≥ 50 µl.


    Cat # Name Size
    D3050-1-5
    C1011-50 Zymo-Spin IIC Columns 50 Pack
    C1001-50 Collection Tubes 50 Pack
    D3067-1-20 Deparaffinization Solution 20 ml
    D3067-2-25 Genomic DNA Wash 1 25 ml
    D3067-3-12 Genomic DNA Wash 2 12 ml
    D3004-1-50 Genomic Lysis Buffer 50 ml
    D3004-4-10 DNA Elution Buffer 10 ml