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    Quick-DNA HMW MagBead Kit


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    Cat # Name Size
    D6060 Quick-DNA HMW MagBead Kit 96 Preps

    Highlights

    • High Molecular Weight DNA: Extract high molecular weight DNA up to 150 kb from any sample.
    • Ultra-Pure: Highest DNA yield and purity equipped with RNA removal technology.
    • Third-Generation Sequencing Ready: Optimized for long read sequencing (including Oxford Nanopore™ and PacBio SMRT™ sequencing).
    Description & Documents Specifications FAQ Components

    Documents

    Product Description

    The Quick-DNA HMW Magbead Kit is the easiest method to purify high molecular weight DNA from any sample (including biological fluids, cell cultures, and solid tissues). The purified HMW DNA is ideal for long read sequencing and immediately ready for third-generation sequencing platforms such as Nanopore and PacBio SMRT Sequencing. With a unique binding system, the Quick-DNA HMW Magbead kit delivers ultra-pure and concentrated genomic DNA up to 150 kb.

    For microbiome samples, we recommend using ZymoBIOMICS DNA Kits.

    Documents

    Technical Specifications

    DNA Purity High quality DNA is ready for all sensitive downstream applications such as long read sequencing, PCR, endonuclease digestion, Southern blotting, genotyping, Next-Generation Sequencing, bisulfite conversion, etc. (A260/A230 ≥ 1.8).
    DNA Size Capable of recovering genomic and mitochondrial DNA sized fragments up to 150 kb. If present, plasmid, parasitic, microbial, and viral DNA will also be recovered.
    DNA Yield The DNA binding capacity is 10 µg per 50 µl MagBinding Beads used.
    Elution Volume 50 µl DNA Elution Buffer per 33 µl MagBinding Beads
    Equipment Magnetic rack, shaker and/or rotator, and microcentrifuge (optional)
    Sample Types Any cells, solid tissue, whole blood, saliva, biological fluids, buccal, swabs, samples stored in DNA/RNA Shield, etc.

    Resources

    DNA profiles generated using this kit range from 20kb – 200kb depending on sample type and handling.

    Yes, you can directly input DNA extracted using this kit into any long-read sequencing workflow.

    To increase the amount of DNA recovered, simply increase the amount of MagBinding Beads and DNA Elution Buffer used in the system (e.g. 100 µl MagBinding Beads eluted in 150 µl DNA elution buffer can bind up to 20-25 µg of HMW DNA).

    ZymoBIOMICS DNA Kits are recommended for processing microbial and environmental samples for long-read metagenomics sequencing.

    Cat # Name Size
    D3004-2-200 g-DNA Wash Buffer 200 ml
    D3004-4-50 DNA Elution Buffer 50 ml
    D3004-5-50 DNA Pre-Wash Buffer 50 ml
    D3001-2-20 Proteinase K w/ Storage Buffer Set 20 mg
    D4100-2-6 MagBinding Beads 6 ml
    D4077-1-150 Quick-DNA MagBinding Buffer 150 ml
    D4081-3-25 Biofluid & Solid Tissue Buffer 25 mL

    “The kit was very user friendly and easy to use. I was very satisfied with the results and the size fragments that the kit yielded.”

    - D. K. (BASF)

    “Great results in terms of yield and integrity of HMW DNA”

    - M.P. (U of Louisville)

    “The yield is good and the size of the extracted DNA is really high.”

    - M. M. (UCLA)

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