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    Quick-DNA Microprep Kit


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    Cat # Name Size
    D3021 Quick-DNA Microprep Kit 200 Preps
    D3020 Quick-DNA Microprep Kit 50 Preps

    Highlights

    • Easy purification of high-quality DNA from whole blood, buffy coat, swabs, or cultured cells.
    • Protocol excludes the use of Proteinase K and organic denaturants for biofluid and cell samples.
    • Eluted, inhibitor-free DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
    Description & Documents Specifications FAQ Components

    Documents

    Supplemental Info

    Product Description

    The Quick-DNA Kits are ideal DNA isolation kits for easy, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), buffy coat, buccal cells, cells from culture, saliva, and other biological liquid samples can be processed with these kits. Zymo-Spin Column/Plate technology enables high-quality DNA purification in minutes. PCR inhibitors are effectively removed, and the eluted DNA is ideal for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.

    Documents

    Technical Specifications

    Elution Volume ≥10 µl
    Equipment Microcentrifuge, Vortex
    Purity High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8
    Sample Source Whole blood, plasma, or serum from humans, mice, rats, etc. Cells from culture, buccal cells, as well as a variety of biological liquids are effectively processed using this kit. Tissue already digested with Proteinase K or mechanically homogenized.
    Size Range Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered
    Workflow Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples.
    Yield Up to 5 µg total DNA is eluted into ≥10 µl (6 µl minimum) DNA Elution Buffer or water. Human whole blood yields 1.5-3.5 µg DNA per 50 µl blood sampled. Mammalian tissues already homogenized will yield 1-3 µg DNA per mg.
    Supplemental Info

    Resources

    The Quick-DNA kit is optimized for cells, soft tissues, and homogenized/digested samples using a single lysis/binding buffer. The Quick-DNA Plus kits contain an optimized Proteinase K for processing a wider variety of sample inputs, such as cells, blood, tissues, etc. The upgraded Quick-DNA Plus typically recovers more DNA.

    White blood cells, which are the major source of genomic DNA in blood, easily and quickly settles. Mix the blood sample well prior to taking an aliquot for purification.

    E.coli cells are easy-to-lyse and can be processed directly. For other microbes, additional pretreatment (e.g. enzymatic digestion or mechanical lysis) can be implemented and then processed with the Quick-DNA Kit. Alternatively, for an all-inclusive kit to process all microbes, use any of Zymo Research’s Environmental Kits (e.g. Quick-DNA Fungal/Bacterial, Quick-DNA Fecal/Soil, ZymoBIOMICS DNA, etc.) for DNA isolation.

    No, the kit is designed for direct use with biological samples. For clean-up of isolated DNA, please use the Genomic DNA Clean & Concentrator or the DNA Clean & Concentrator kits.

    Yes, add 4 volumes of Genomic Lysis Buffer to 1 volume of crude lysate, homogenized, or digested sample (see Cell Suspensions and Proteinase K Digested Samples) and proceed with the remainder of the protocol.

    Beta-mercaptoethanol is a reducing agent that helps break down proteins and improves DNA recovery and purity. Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM) or omitted.

    The Quick-DNA kits recover RNA-free genomic DNA. The selective chemistry allows for binding of double stranded DNA to the column and for RNA to flow through. No RNase A treatment is required when processing samples within kit specifications.

    Keep in mind that there is sample to sample variability.

    Sample Type Input Amount Expected Yield
    Eukaryotic Cells 1x106 Cells 5-6 µg
    Skeletal, Heart, Lung, Brain Tissue 1 mg 1-3 µg
    Liver and Kidney Tissue 1 mg 3-5 µg
    Human Whole Blood 100 µl 5-7 µg

    Cat # Name Size
    D3004-4-10 DNA Elution Buffer 10 ml
    D3004-2-100 g-DNA Wash Buffer 100 ml
    D3004-2-50 g-DNA Wash Buffer 50 ml
    D3004-1-100 Genomic Lysis Buffer 100 ml
    D3004-1-50 Genomic Lysis Buffer 50 ml
    D3004-5-15 DNA Pre-Wash Buffer 15 ml
    D3004-5-50 DNA Pre-Wash Buffer 50 ml
    C1001-50 Collection Tubes 50 Pack
    C1004-50 Zymo-Spin IC Columns 50 Pack

    “It was easy to work with, protocol easy to follow”

    - Tinatin T.

    “This kit did a good job of prepping clean genomic DNA.”

    - Tara N. (United States Agricultural Research Service)

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