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    Quick-DNA Plant/Seed 96 Kit


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    Cat # Name Size
    D6021 Quick-DNA Plant/Seed 96 Kit 2 x 96 Preps

    Highlights

    • Rapid, simple, high-throughput (96-well) method for DNA isolation from tough-to-lyse plant and seed samples.
    • Ultra-high density BashingBeads are fracture resistant and chemically inert.
    • Zymo-Spin technology coupled with filtration removes polyphenolic PCR inhibitors from the DNA product.
    Description & Documents Specifications FAQ Components

    Documents

    Supplemental Info

    Product Description

    The Quick-DNA Plant/Seed 96 Kit is a plant DNA isolation kit designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in minutes: plant samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Polysaccharides, lipids, and polyphenols/tannins are removed from the DNA using our Zymo-Spin technology. The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, etc.

    Documents

    Technical Specifications

    Applicable For All sensitive downstream applications such as qPCR and Next-Generation sequencing.
    Elution Volume ≥ 50 µl
    Equipment  Centrifuge w/ microplate carriers, 96-well plate/block disruptor or pulverizer.
    Processing Volume ≤ 80 mg
    Purity A260/A280 nm ≥1.8.
    Sample Source Leaves, stems, buds, flowers, fruit, seeds, etc.
    Sample Storage DNA stored at ≤ -20°C.
    Size Range Capable of recovering genomic DNA up to and above 40 kb. Typical fragment sizes range from 25 kb - 35 kb. If present, parasitic and viral DNA will also be recovered.
    Type Total DNA
    Yield ≤ 5 µg total DNA
    Supplemental Info

    Resources

    We currently do not have a list of plants, aside from what we have shown in the protocols: – A.thaliana – Juniper – Milkweed Leaf – Milkweed Leaflet – Milkweed Pre-Flowering Bud – Corn Kernel – Sunflower Seed – Nicotiana sp.

    A viscous sample can indicate incomplete sample lysis. Try using less of your sample and optimize bead beating conditions (duration, speed, time) to ensure samples are thoroughly lysed. After bead beating, pellet the cell debris before moving on. Adding more Genomic Lysis buffer to the lysate can help dilute and deproteinate the sample, making the sample less viscous and more suitable for DNA recovery.

    Environmental samples often contain inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanins, etc. that often co-purify and affect downstream applications such as PCR. The Zymo-Spin III-HRC column removes these polyphenolic PCR inhibitors to recover DNA that is ready for sensitive downstream applications such as Next-Gen Sequencing, quantitative PCR, etc.

    Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM). However, if bead beating is optimized and lysis is efficient, the addition of BME is not necessary and can be omitted.

    The Quick-DNA kits recover RNA-free genomic DNA. The selective chemistry allows for binding of double stranded DNA to the column and for RNA to flow through. No RNase A treatment is required when processing samples within kit capacity.

    Cat # Name Size
    S6002-96-2 ZR-96 BashingBead Lysis Rack 2 mm
    C2009 Silicon-A-HRC Plate 2 Plates
    C2003 Elution Plate 2 Plates
    C2002 Collection Plate 2 Plates
    C2007-4 96-Well Plate Cover Foil 4 Foils
    P1001-2 96-Well Block 2 Blocks
    C2001 Silicon-A Plate 2 Plates
    D6035-1-30 Prep Solution 30 ml
    D6001-3-40 BashingBead Buffer 40 ml
    D3004-5-50 DNA Pre-Wash Buffer 50 ml
    D3004-4-10 DNA Elution Buffer 10 ml
    D3004-2-100 g-DNA Wash Buffer 100 ml
    D3004-1-150 Genomic Lysis Buffer 150 ml

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