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Quick-DNA/RNA™ Water Kit
Cat # | Name | Size |
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Highlights
- Robust Total DNA/RNA Recovery: Concentrate and purify total DNA/RNA from large volume water samples including viral, pathogen, microbial & free nucleic acids.
- Superior Inhibitor Removal: Innovative inhibitor removal technology that yields DNA/RNA from the toughest water samples including raw wastewater, sludge, etc.
- Analytic Compatibility: Eluted DNA/RNA is ready for any sensitive downstream applications including ddPCR, RT-qPCR, NGS, etc.
Documents
Product Description
Technical Specifications
Sample Sources | Water, wastewater, sewage, sludge, finished water, natural water, river water, fresh water, salt water, etc. |
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Sample Size | • Up to 1 L low biomass liquid samples • Up to 50 mL raw wastewater sample |
DNA/RNA Purity | High quality, inhibitor-free DNA/RNA (A260/A280 > 1.8, A260/A230 > 1.8) suitable for all downstream applications including NGS, qPCR, ddPCR, RT-qPCR, and RT-ddPCR. |
Yield | Up to 25 µg DNA or 100 µg RNA eluted into ≥ 50 µl |
DNA/RNA Storage | DNA and/or RNA is eluted with Inhibitor Removal Elution Buffer and can be stored at ≤ -70°C. |
Equipment Needed (user provided) | Microcentrifuge, vortex, floor model centrifuge capable of spinning 50 ml conical tubes, magnetic stand1, and rotator capable of at least 30 rpm. |
Resources
Q1: Can I use this kit with filters or other concentrated water samples?
Yes. This kit can be used to extract DNA/RNA from water filters, molecular cut off filters, wastewater solids, sludge, and other concentrated water sample sources. See Section I: Sample Preparation of the instruction manual for specific processing instructions.
Q2: What equipment is required to perform this protocol?
Microcentrifuge, vortex, floor model centrifuge capable of spinning 50 ml conical tubes, magnetic stand, and rotator capable of at least 30 rpm.
Q3: Can I perform a DNase treatment to recover only RNA?
Yes. An optional DNase treatment can be performed during Section III: DNA/RNA Purification. Detailed instructions are provided in the appendices of the instruction manual.
Cat # | Name | Size | |
---|---|---|---|
R1100-50 | DNA/RNA Shield | 50 ml | |
R1100-250 | DNA/RNA Shield | 250 ml | |
D7020-1-25 | Viral DNA/RNA Buffer | 25 ml | |
D7020-1-100 | Viral DNA/RNA Buffer | 100 ml | |
D7010-1-10 | DNA/RNA Binding Buffer | 10 ml | |
D7010-1-25 | DNA/RNA Binding Buffer | 25 ml | |
D7010-1-50 | DNA/RNA Binding Buffer | 50 ml | |
D7010-2-10 | DNA/RNA Prep Buffer | 10 ml | |
D7010-2-25 | DNA/RNA Prep Buffer | 25 ml | |
D7010-2-50 | DNA/RNA Prep Buffer | 50 ml | |
D7010-2-200 | DNA/RNA Prep Buffer | 200 ml | |
D7010-3-6 | DNA/RNA Wash Buffer (Concentrate) | 6 ml | |
D7010-3-12 | DNA/RNA Wash Buffer (Concentrate) | 12 ml | |
D7010-3-24 | DNA/RNA Wash Buffer (Concentrate) | 24 ml | |
W1001-1 | DNase/RNase-Free Water | 1 ml | |
W1001-4 | DNase/RNase-Free Water | 4 ml | |
W1001-6 | DNase/RNase-Free Water | 6 ml | |
W1001-10 | DNase/RNase-Free Water | 10 ml | |
C1006-50-G | Zymo-Spin IIICG Columns | 50 Pack | |
C1006-250-G | Zymo-Spin IIICG Columns | 250 Pack | |
E1010 | DNase I Set | 250 U | |
E1011 | DNase I Set | 1500 U | |
E1012 | DNase I Set | 5 x 1500 U | |
C1001-50 | Collection Tubes | 50 Pack | |
C1001-500 | Collection Tubes | 500 Pack | |
C1001-1000 | Collection Tubes | 1000 Pack | |
S6012-50 | ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) | 50 Tubes | |
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