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    Quick-RNA Fecal/Soil Microbe Microprep Kit


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    Cat # Name Size
    R2040 Quick-RNA Fecal/Soil Microbe Microprep Kit 50 preps

    Highlights

    • High Quality: Extract intact high quality total RNA (including small/micro RNAs) from various soil, fecal, and water samples.
    • Homogenization: Ultra-high density BashingBead technology for complete disruption of tough-to-lyse samples.
    • Inhibitor-Free: RNA is ready for all downstream applications including sequencing, RT-PCR, microarray, etc.
    Description & Documents Specifications Components

    Documents

    Product Description

    The Quick-RNA Soil/Fecal Microbe Microprep is an innovative fecal and soil RNA extraction kit designed for the simple, reliable, and rapid isolation of total RNA including small RNAs (>17 nt) from various soil, sludge, sediment and/or fecal samples. The procedure of this fecal and soil RNA extraction kit successfully isolates RNA from tough-to-lyse bacteria, fungi, protozoa (protist), algae, etc. in soil, and host RNA from fecal samples. Samples are added to the ZR BashingBead Lysis Tube with an optimally designed S/F RNA Lysis Buffer where microbes are then lysed by bead beating to extract total RNA. The Zymo-Spin column technology included in this fecal and soil RNA extraction kit allows for quick filtration, genomic DNA removal from sample lysates, and isolation of the RNA. Zymo-Spin III-HRC Spin Filter separates RT-PCR inhibitors (e.g., humic acids, polyphenols, tannins) and the total RNA is concentrated using the Zymo-Spin IC Column with a minimum elution volume of ≥6 µl.

    Documents

    Technical Specifications

    Equipment Microcentrifuge, vortex and/or cell disrupter/pulverizer (optional)
    Purity RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
    Sample Source Bacteria, fungi, protozoa, and algae in up to 250 mg soil, sludge or sediments, and bacteria, protist and/or host RNA from feces (mammalian, avian, etc.)
    Size Range Total RNA ≥ 17 nt
    Yield 10 µg RNA (binding capacity), ≥6 µl (elution volume)

    Resources

    Cat # Name Size
    W1001-6 DNase/RNase-Free Water 6 ml
    C1004-50 Zymo-Spin IC Columns 50 Pack
    C1006-50-G Zymo-Spin IIICG Columns 50 Pack
    R1060-2-25 RNA Prep Buffer 25 ml
    R2040-1-50 S/F RNA Lysis Buffer 50 ml
    R1013-2-50 RNA Binding Buffer 50 ml
    R1003-3-24 RNA Wash Buffer 24 ml
    S6012-50 ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) 50 Tubes
    C1058-50 Zymo-Spin III-HRC Filters 50 Pack

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