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    Quick-RNA Midiprep Kit


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    Cat # Name Size
    R1056 Quick-RNA Midiprep Kit 25 preps

    Highlights

    • Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
    • High Yield: Recover up to 1 mg of RNA
    • NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
    Description & Documents Specifications FAQ Components

    Documents

    Supplemental Info

    Product Description

    The Quick-RNA Midiprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of up to 1 mg total RNA from cultured cells or tissue samples. The procedure combines a unique, single-step RNA extraction/binding buffer with Clean-Spin™ column technology to yield high quality RNA in about 10 minutes. The Quick-RNA™ Midiprep Kit allows for the efficient recovery of total RNA from 103 to 108 cells or tissue. The method is easy: simply add the provided ZR RNA Buffer to extract total RNA from the cells of interest then purify the RNA using the provided Zymo-Spin™ V-E Columns. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.

    Learn More

    Documents

    Technical Specifications

    Equipment Microcentrifuge, vortex, vacuum manifold
    Purity RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
    Sample Source Cells or tissue samples, yeast, plant or bacteria.
    Size Range Total RNA ≥ 17 nt
    Yield Midiprep: 1 mg RNA (binding capacity), ≥ 200 µl (elution volume)
    Supplemental Info

    Resources

    Yes, the catalog number for the DNase I set (DNase and DNA Digestion Buffer) that we offer is E1010.

    If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.

    Yes. Use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.

    Yes, this kit will isolate small/micro RNA’s ≥ 17 nucleotides.

    Cat # Name Size
    W1001-10 DNase/RNase-Free Water 10 ml
    C1001-50 Collection Tubes 50 Pack
    R1003-3-6 RNA Wash Buffer 6 ml
    R1020-1-100 ZR RNA Buffer 100 ml
    R1020-2-12 RNA Pre-Wash Buffer 12 ml

    "RNA isolation from tissue culture cells used to be my most hated protocol - labor intensive, tedious, and the horrible smell of the BME used in the protocol. This kit is much faster and easier and no horrible smells! Both the RNA yield and the real time results are just as good as our previous kit."

    - Lisa G. (University of Virginia)

    “Amazing results, the only RNA extraction kit I ever buy now. In my opinion, there is no product on the market that is as good a value and as effective as this kit for plant tissue RNA extractions.”

    - Erin B. (Occidental College)

    "Good price and easy to use, plus good quality. It is compatible to QIAGEN products RNeasy kit, but with reasonable price and equal quality. Especially the DNase is inexpensive and is included in the kit. No matter animal RNA, or plant RNA, or bacterial RNA, all could use with this one kit."

    - Xiaolu J. (Florida Atlantic University)

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