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    Quick-RNA Plant Kit


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    Cat # Name Size
    R2024 Quick-RNA Plant Miniprep Kit 50 preps

    Highlights

    • High Quality: Extract intact high quality total RNA (including small/micro RNAs) from a wide variety of plant samples.
    • Homogenization: Ultra-high density BashingBead technology for complete disruption and sample lysis.
    • Inhibitor-Free: RNA is ready for all downstream applications including sequencing, RT-PCR, microarray, etc.
    Description & Documents Specifications Components

    Documents

    Product Description

    The Quick-RNA Plant Miniprep Kit provides for rapid isolation of RNA from various plant samples (e.g. leaves, stems, buds, flowers, fruit, seeds etc.). For purification of total RNA including small RNAs (~50 µg), the Quick-RNA Plant Miniprep features a specially formulated RNA Lysis Buffer. The Zymo-Spin IIICG Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin IIC Column efficiently adsorbs total RNA. The RNA is washed and then eluted with DNase/RNase-Free Water. For inhibitor removal, the eluted RNA can be treated by running the sample through the Zymo-Spin III-HRC Spin Filters. The RNA is suitable for use in various subsequent procedures including RT-PCR.

    Documents

    Technical Specifications

    Equipment Microcentrifuge, vortex and/or cell disrupter/pulverizer (optional)
    Purity RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
    Sample Source Leaves, stems, buds, flowers, fruit, seeds, etc.
    Size Range Total RNA ≥ 17 nt
    Yield 50 µg RNA (binding capacity), ≥25 µl (elution volume)

    Resources

    Cat # Name Size
    W1001-4 DNase/RNase-Free Water 4 ml
    C1006-50-G Zymo-Spin IIICG Columns 50 Pack
    C1001-50 Collection Tubes 50 Pack
    R1060-2-25 RNA Prep Buffer 25 ml
    R1060-1-50 RNA Lysis Buffer 50 ml
    R1060-1-100 RNA Lysis Buffer 100 ml
    R1003-3-12 RNA Wash Buffer 12 ml
    S6003-50 ZR BashingBead Lysis Tubes (2 mm) 50 Tubes
    C1078-50 Zymo-Spin IICR Columns 50 Pack
    C1058-50 Zymo-Spin III-HRC Filters 50 Pack

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