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    YeaStar RNA Kit


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    Cat # Name Size
    R1002 YeaStar RNA Kit 40 Preps

    Highlights

    • Simple: Fast spin-column procedure yields pure yeast RNA without using glass beads or phenol.
    • Versatile: Efficient RNA isolation from a broad spectrum of fungal species susceptible to Zymolyase.
    • High-Quality: Isolated RNA is suitable for use in RT-PCR, Northern blotting, etc.
    Description & Documents Specifications FAQ Components Citations

    Documents

    Product Description

    The YeaStar RNA Kit provides all the necessary reagents for RNA isolation from a broad spectrum of fungi including: Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans/, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe. Generally, the kit can be used for the purification of high-quality, total RNA from any fungus that can be lysed by yeast lytic enzyme. The kit facilitates the purification of 10-25 µg of total RNA from 1-1.5 ml of cultured cells using innovative Zymo-Spin column technology.

    Documents

    Technical Specifications

    Applicable For Isolated RNA is well suited for downstream applications such as Northern blotting, RT-PCR, Next-Generation Sequencing, Microarray, hybridization and other sensitive applications
    Elution Volume ≥ 60 µl DNase/RNase-Free Water
    Equipment Microcentrifuge & Heat Block/Bath
    Processing Time 30 minutes
    Processing Volume ≤ 1.5 ml of Culture (1-5 x 107 cells)
    Purity Typical A260/A280 & A260/A230 ≥ 1.8
    Sample Source Cell Culture (Colonies/Patches or Liquid Culture)
    Size Range ≥ 17 nt
    Type Total RNA
    Yield ≤ 25 µg of total RNA

    Resources

    Any strains susceptible to Zymolyase, which includes the following fungal genera: Asbya Kloekera Candida Kluyveromyces Debaryomyces Lipomyces Eremothecium Metschikowia Endomyces Pichia Hansenula Pullularia Hanseniaspora Saccharomyces Saccaromycodes Saccharomycopsis Schizosaccahromyces Torulopsis

    Cat # Name Size
    W1001-6 DNase/RNase-Free Water 6 ml
    R1001-1 YR Digestion Buffer 3.2 ml
    R1001-2 YR Lysis Buffer 6.4 ml
    R1003-3-6 RNA Wash Buffer 6 ml
    C1001-50 Collection Tubes 50 Pack
    C1006-50-G Zymo-Spin IIICG Columns 50 Pack
    E1004 Zymolyase 1000 U

    RT-PCR was used to analyze S1 nuclease protection using RNA extracted from Saccharomyces cerevisiae strains with the YeaStar RNA kit. Researchers showed that histone H2B monoubiquitylation and it deubiquitylation are related in gene activation.

    Henry, KW et al. Transcriptional activation via sequential histone H2B ubiquitylation and deubiquitylation, mediated by SAGA-associated Ubp8. Genes & Development. 2003.

    The YeaStar RNA kit was used to extract total RNA from Saccharomyces cerevisiae cells and spheroblasts for downstream qRT-PCR quantification. The WEE1 kinase was found to be an epigenetic modulator that inhibits transcription of histone genes.

    Mahajan, K et al. H2B Tyr37 phosphorylation suppresses expression of replication-dependent core histone genes. Nature Structural & Molecular Biology. 2012.

    Researchers found that two positive feedback loops regulated by Gal1p and Gal3p affect network activity and induce bimodal distribution of gene expression. The YeaStar RNA kit was used for yeast RNA extraction for real-time qPCR.

    Venturelli, OS et al. Synergistic dual positive feedback loops established by molecular sequestration generate robus bimodal response. PNAS. 2012.

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