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    ZR-96 DNA Clean-Up Kit


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    Cat # Name Size
    D4017 ZR-96 DNA Clean-Up Kit (Shallow Well) 2 x 96 Preps
    D4018 ZR-96 DNA Clean-Up Kit (Shallow Well) 4 x 96 Preps

    Highlights

    • Quick (20 minute), large-scale recovery of ultra-pure DNA from PCR, endonuclease digestions, cell-free lysates, etc.
    • ZR-96 Silicon-A Plate design allows DNA to be eluted at high concentrations into minimal volumes of solvent.
    • Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc.
    Description & Documents Specifications FAQ Components

    Documents

    Product Description

    The ZR-96 DNA Clean-up Kit is a PCR purification kit that provides for rapid, large-scale (96-well) purification and concentration of high-quality DNA from PCR samples, endonuclease digestions, or crude plasmid preparations. Simply add the specially formulated DNA Binding Buffer to your samples and transfer to the wells of the supplied Silicon-A Plate. There is no need for organic denaturants or chloroform. Instead, the product features Fast-Spin plate technology to yield high-quality, purified DNA in just minutes.

    Documents

    Technical Specifications

    Detergent Tolerance ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS
    Elution Volume ≥ 30 µl for shallow well, ≥ 10 µl for deep well
    Equipment Centrifuge with microplate carriers
    Purity A260/A280 > 1.8
    Sample Source DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.
    Size Range 75 bp to 23 kb for shallow well, 50 bp to 23 kb for deep well
    Yield ≤ 5 µg total DNA can be recovered. For DNA 75 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.

    Resources

    Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.

    Add an equal volume of ethanol (95-100%) to the sample and mix well. The sample is ready-to-bind and does not require DNA Binding Buffer. Proceed to Step 2.

    The DNA will be eluted off the column. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.

    Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.

    We recommend no more than 5 times as binding efficiency might decrease.

    Working with volumes below 50 µl can result in decreased recovery. We recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions.

    Cat # Name Size
    D4003-2-24 DNA Wash Buffer (Concentrate) 24 ml
    D4004-1-L DNA Binding Buffer 100 ml
    D4003-2-48 DNA Wash Buffer (Concentrate) 48 ml
    C2001 Silicon-A Plate 2 Plates
    C2003 Elution Plate 2 Plates
    C2002 Collection Plate 2 Plates

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