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Read The Zymo Research PromiseZR BAC DNA Miniprep Kit
| Cat # | Name | Size |
|---|
Highlights
- High Purity: Purify endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, restriction endonuclease digestion, etc.
- Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization
- Unique column design: Zero buffer retention and low elution volume (10 µl)
Documents
Product Description
Technical Specifications
| Applicable For | Transfection, transformation, sequencing, restriction endonuclease digestion, PCR, in vitro transcription, and other sensitive applications. |
|---|---|
| Elution Volume | ≥ 10 µl |
| Equipment | Microcentrifuge |
| Processing Time | 15 min |
| Purity | Typical Abs 260/280 ≥ 1.8 and Abs 260/230 ≥ 2.0 |
| Size Range | Up to ~200 kb |
| Yield | Up to 10 µg per preparation, depending on the plasmid copy number, initial volume of E. Coli culture processed, and culture growth conditions. The typical yield is 1 µg per 3 ml of culture. |
Resources
Q1: I accidently left my P3 Buffer at room temperature, is it still okay to use?
Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4 °C as soon as possible to ensure optimal performance throughout the life span of the product.
| Cat # | Name | Size | |
|---|---|---|---|
| D3004-4-16 | DNA Elution Buffer | 16 ml | |
| D3004-4-1 | DNA Elution Buffer | 1 ml | |
| D4036-3-60 | Endo-Wash Buffer | 60 ml | |
| D4036-3-15 | Endo-Wash Buffer | 15 ml | |
| D4036-3-30 | Endo-Wash Buffer | 30 ml | |
| D4036-3-6 | Endo-Wash Buffer | 6 ml | |
| D4027-1-10 | Buffer P1 (Red) | 10 ml | |
| D4027-1-160 | Buffer P1 (Red) | 160 ml | |
| D4027-1-80 | Buffer P1 (Red) | 80 ml | |
| D4027-2-10 | Buffer P2 (Green) | 10 ml | |
| D4027-1-20 | Buffer P1 (Red) | 20 ml | |
| D4027-4-6 | Plasmid Wash Buffer | 6 ml | |
| D4027-4-48 | Plasmid Wash Buffer | 48 ml | |
| D4027-4-24 | Plasmid Wash Buffer | 24 ml | |
| D4027-3-50 | Buffer P3 (Yellow) | 50 ml | |
| D4027-4-12 | Plasmid Wash Buffer | 12 ml | |
| D4027-3-220 | Buffer P3 (Yellow) | 220 ml | |
| D4027-3-440 | Buffer P3 (Yellow) | 440 ml | |
| D4027-2-80 | Buffer P2 (Green) | 80 ml | |
| D4027-3-12 | Buffer P3 (Yellow) | 12 ml | |
| D4027-2-20 | Buffer P2 (Green) | 20 ml | |
| D4027-2-160 | Buffer P2 (Green) | 160 ml | |
| D3004-4-4 | DNA Elution Buffer | 4 ml | |
| C1001-50 | Collection Tubes | 50 Pack | |
| C1002-25 | Zymo-Spin IC-XL | 25 Pack | |
| C1002-50 | Zymo-Spin IC-XL | 50 Pack | |
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