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    Zymo Environ Water RNA Kit


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    Cat # Name Size
    R2042 Zymo Environ Water RNA Kit 50 preps

    Highlights

    • Enrich total RNA from large volumes (≤ 5 ml) and transform into small sample pellets for easy processing.
    • Inactivates infectious agents and pathogens (viruses, bacteria, fungi, and parasites) for safe handling using DNA/RNA Shield.
    • Remove PCR inhibitors in one spin and elute concentrated RNA in ≥6 µl. Ready for any downstream application including RT-qPCR, RTddPCR, NGS.
    Description & Documents Specifications Components

    Documents

    Product Description

    The Zymo Environ Water RNA Kit simplifies COVID-19 wastewater surveillance by increasing viral enrichment, inactivating pathogens, and improving limits of detection. The use of Water Concentrating Buffer (WCB) promotes the pelleting of viral capsids and protein-associated RNA, increasing SARS-CoV-2 RNA yields by more than 8-fold. DNA/RNA Shield allows for safe handling as it has been validated for inactivation of pathogens including SARS-CoV-2. Finally, the workflow yields highly concentrated, PCR inhibitor-free RNA ready for downstream applications including RT-qPCR, RT-ddPCR, and NGS. Learn more.

    Documents

    Technical Specifications

    Equipment Needed Microcentrifuge, vortex, large 50 mL conical tube centrifuge (optional), cell disrupter/pulverizer (optional)
    Format Bead beating, spin column.
    RNA Purity High quality RNA (A260/A280 > 1.8, A260/A230 > 1.8) suitable for all downstream RNA-based manipulations.
    RNA Storage RNA is eluted with RNase-free water and can be stored at ≤-70°C. The addition of RNase inhibitors is highly recommended for prolonged storage.
    Sample Inactivation DNA/RNA Shield™ lyses cells, inactivates nucleases and infectious agents, and is ideal for safe sample storage and transport at ambient temperatures.
    Sample Input Water, wastewater, sludge, sewage, biofilms, etc.
    Sample Size Up to 5 mL
    Yield Up to 10 µg RNA can eluted into ≥ 6 µL RNase-free water allowing for a highly concentrated sample.

    Resources

    Cat # Name Size
    R1100-50 DNA/RNA Shield 50 ml
    W1001-6 DNase/RNase-Free Water 6 ml
    D6035-1-30 Prep Solution 30 ml
    R1003-3-24 RNA Wash Buffer 24 ml
    R1013-2-50 RNA Binding Buffer 50 ml
    R1060-2-25 RNA Prep Buffer 25 ml
    C1004-50 Zymo-Spin IC Columns 50 Pack
    C1006-50-G Zymo-Spin IIICG Columns 50 Pack
    C1058-50 Zymo-Spin III-HRC Filters 50 Pack
    S6012-50 ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) 50 Tubes
    R2042-1-8 Water Concentrating Buffer 8 mL

    “Reliable results and very easy to use. Have been working on wastewater SARS-CoV-2 surveillance for over 9 months now. Tried several methods and kits for concentration of virus particles in wastewater. This kit from Zymo was one of the top performers. What makes it unique is the water concentrating buffer. Will strongly recommend this product."


    - Shireen K, University of Virginia, SARS-CoV-2 Surveillance

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