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Read The Zymo Research PromiseZymo-Seq Methyl Spike-in Control
| Cat # | Name | Size |
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Highlights
- Ensures reliable calculation of bisulfite conversion efficiency post library prep.
- Six unique amplicons with 0, 10, 25, 50, 75, and 100% methylation levels allow for a standard curve and robust data normalization.
- Compatible with various species (except for E. coli) and bisulfite sequencing library preparation methods.
Documents
Product Description
Technical Specifications
| Concentration | 60 pg/µl | ||||||||||||||
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| Methylation Levels |
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| Source | Double-stranded synthetic amplicons derived from the E. coli genome. | ||||||||||||||
| Storage | ≤ 20°C |
Resources
Q1: What percentage of reads are assigned to the Zymo-Seq Methyl Spike-in Control in sequencing?
Less than 0.5% of the total reads goes into the Zymo-seq Methyl Spike-in Control.
Q2: How much Zymo-Seq Methyl Spike-in Control should be added to DNA samples for library preparation?
We recommend using 60pg of Zymo-Seq Methyl Spike-in Control for 100-500ng of sample DNA, 30pg for 50-99ng of DNA, and 10pg for 20-49ng of DNA. Depending on the required amount of the Zymo-Seq Methyl Spike-in Control per DNA sample, it can be further diluted to the appropriate concentration in TE buffer.
Q3: Where can I get the reference sequence of the Zymo-Seq Methyl Spike-in Control for mapping?
The reference sequence of each amplicon can be found in the instruction manual.
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