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    Zymo-Seq RiboFree Universal cDNA Kit


    Zymo Research's 100% Satisfaction Guarantee

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    Read The Zymo Research Promise

    This product was updated recently. Refer to FAQ-Q6 for details.

    Cat # Name Size
    R3001 Zymo-Seq RiboFree Universal cDNA Kit 12 Preps

    Highlights

    • Universal Depletion: Novel probe-free technology depletes rRNA from any organism.
    • Simplest Kit: Prepare NGS-grade RiboFree cDNA from total RNA in as little as 1.75 hours.
    • Automation Friendly: Streamlined protocol for increased scalability.
    Description & Documents Specifications FAQ Components

    Documents

    Product Description

    The Zymo-Seq RiboFree Universal cDNA Kit is the simplest kit for the preparation of NGS-grade first-strand cDNA with ribosomal RNA (rRNA) depleted. This simple cDNA kit features RiboFree Universal Depletion, a novel probe-free rRNA depletion technology that depletes rRNA from any organism. The RiboFree Universal Depletion is validated across biological kingdom and phyla, including mammals, plants, and various prokaryotes, and also RNA from a wide range of sample types including cells, snap-frozen tissues, and FFPE samples. The RiboFree single-stranded cDNA produced by the kit can be used for qPCR detection, RNA-Seq library prep, and any other downstream analysis. In contrast to poly(A) enrichment methods, the RiboFree Universal cDNA Kit produces single-stranded cDNA from nearly all RNA biotypes, including full-length mRNA, mRNA with degraded poly(A) tails, long-noncoding RNA (lncRNA), intronic RNA, nucleolar RNA, etc. The cost-effective Zymo-Seq RiboFree Universal cDNA Kit has been optimized to reduce hands-on time, with all the reagents such as SPRI beads included for researchers’ convenience.

    Documents

    Technical Specifications

    1Equipment Needed (user provided) Thermal cycler with heated lid, magnetic stand for 0.2 mL PCR tubes, microcentrifuge for 0.2 mL PCR tubes and 1.5 mL microcentrifuge tubes, and a benchtop vortex mixer.
    2Sample Input Material RNA
    3RNA Input 10 – 1000 ng of total RNA.

    • For optimal results, please use the recommended 10-1000 ng input.
    • If an input below 10 ng is necessary, see Appendix B of the kit protocol for additional considerations and recommended modifications.
    4Input Quality RNA should be free of DNA contamination and enzymatic inhibitors, with A260/A280 and A260/A230 ≥ 1.8. RNA with lower purity ratios (A260/A280 and A260/A230) should be treated with DNase I and purified with the RNA Clean & Concentrator™ (Cat. No. R1013) prior to processing. RNA should be suspended in water, TE, or a low-salt buffer.

    • For optimal results, please use intact RNA (RNA Integrity Number or RIN ≥ 8.0) whenever possible.
    • For degraded RNA input, see Appendix C of the protocol for additional considerations and recommended modifications.
    5Processing Time As little as 1.75 hours

    Resources

    No, the final product is single-stranded cDNA.

    Yes, this is included in the Zymo-Seq RiboFree Total RNA Library Kit.

    This is compatible with any library prep method that uses single-stranded DNA as an input.

    Yes, this kit will work with any organism.

    It is possible to prepare NGS-grade cDNA with input lower than 10 ng, though the performance may be negatively impacted. For modifications of the protocol when using 1-9 ng of RNA as input, please refer to Appendix B in the kit protocol for detailed suggestions or contact tech@zymoresearch.com.

    There are many ways for you to be certain about which instruction manual to follow. One way is to check the cold reagent names: they are different between the two versions, and the updated version includes a reagent called "Depletion Reagent 4". Overall, compared to v1.1.0, the updated version has a lower minimum input (10 ng total RNA) and a shorter depletion reaction time (1 hour for 100 ng of input). To learn more of the kit features since the update, or if you need an electronic copy of the previous instruction manual (v1.1.0), please contact tech@zymoresearch.com.

    Cat # Name Size
    R3004-1-6 Zymo-Seq Wash Buffer 6 mL
    R3004-1-48 Zymo-Seq Wash Buffer 48 mL
    D3004-4-10 DNA Elution Buffer 10 ml
    D3004-4-50 DNA Elution Buffer 50 ml
    W1001-1 DNase/RNase-Free Water 1 ml
    W1001-4 DNase/RNase-Free Water 4 ml
    W1001-6 DNase/RNase-Free Water 6 ml
    W1001-10 DNase/RNase-Free Water 10 ml
    W1001-30 DNase/RNase-Free Water 30 ml
    3DP-1002 PCR Strip MagStand 1 unit

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