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Q1: Are these individual primers or pre-mixed primer sets?
Each tube contains a pre-mixed forward and reverse primer set that contain a unique i5 and i7 index, respectively. The concentration of each UDI primer pre-mix is 5 µM total (2.5 µM each primer).
Q2: What are the sequences of the indexes?
Please view the “Zymo-Seq UDI Primer Set 1-96” spreadsheet for index sequences and adapter trimming parameters.
Q3: Will this work with Nextera adapters?
No, these index primers only work with TruSeq compatible libraries.
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