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    Zymo-Spin ChIP Kit


    Zymo Research's 100% Satisfaction Guarantee

    Satisfaction Guaranteed

    Read The Zymo Research Promise

    Cat # Name Size
    D5209 Zymo-Spin ChIP Kit 10 Preps
    D5210 Zymo-Spin ChIP Kit 25 Preps

    Highlights

    • Robust immunoprecipitation and purification of DNA.
    • Unique workflow features a micro-elution (≥6 µl) spin column for purification of ChIP DNA.
    • High-quality ChIP DNA is ideal for ChIP-qPCR, ChIP-Seq, and other molecular applications.
    Description & Documents Specifications Components Citations

    Documents

    Product Description

    The Zymo-Spin ChIP Kit from Zymo Research provides a streamlined ChIP procedure for investigating protein-DNA interactions that have been “fixed” in their natural state and can be used to effectively identify binding sites for transcription factors, co-factors, and other DNA regulatory proteins.

    Documents

    Technical Specifications

    Applicable For Chromatin Immunoprecipitation
    Sample Source Mammalian Cells
    Storage Upon arrival, ZymoMag Protein A should be stored at 4°C. All other components, including lyophilized Proteinase K, should be stored at room temperature. Once reconstituted, Proteinase K should be stored at ≤ -20°C.

    Resources

    Cat # Name Size
    D3004-4-10 DNA Elution Buffer 10 ml
    D3004-4-16 DNA Elution Buffer 16 ml
    D4003-2-6 DNA Wash Buffer (Concentrate) 6 ml
    D5201-1-50 ChIP DNA Binding Buffer 50 ml
    M2001 ZymoMag Protein A 200 µl
    M2001-2 ZymoMag Protein A 400 µl
    D5210-1-30 Chromatin Shearing Buffer 30 ml
    D5210-2-30 Chromatin Dilution Buffer 30 ml
    D5210-3-30 Chromatin Wash Buffer I 30 ml
    D5210-4-30 Chromatin Wash Buffer II 30 ml
    D5210-5-30 Chromatin Wash Buffer III 30 ml
    D5210-6-10 5X Chromatin Elution Buffer 10 ml
    D5210-7-1 5M NaCl 1 ml
    D5220-2-30 Nuclei Prep Buffer 30 ml

    “An excellent product that is easy to use.”

    - A. A. – Karmanos Cancer Institute

    “The kit reduced the amount of time needed for my ChIP experiments, the DNA purification was more efficient than other kits, and the purity of the DNA was higher. The endpoint results generated were highly informative to my experimental objectives.”

    - F. E. – Cleveland Clinic

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