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    ZymoBIOMICS Spike-in Control II (Low Microbial Load)


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    Cat # Name Size
    D6321 ZymoBIOMICS Spike-in Control II (Low Microbial Load) 25 Preps
    D6321-10 ZymoBIOMICS Spike-in Control II (Low Microbial Load) 250 Preps

    Highlights

    • Absolute Quantification: Enables cell number measurements from low bacterial load samples using Next-Gen Sequencing.
    • In situ Quality Control: Ensures each sample is quantified accurately.
    • Log Abundance Distribution: Three microbes alien to the human microbiome in log-distribution from 103 to 105 cells.
    Description & Documents Specifications FAQ Related Products

    Documents

    Supplemental Info

    Product Description

    ZymoBIOMICS Spike-in Control II (Low Microbial Load) consists of three bacteria strains, Truepera radiovictrix, Imtechella halotolerans and Allobacillus halotolerans. When spiked into a microbial sample, this product will serve as an in situ positive control for DNA-sequencing-based microbiome measurements. Of the three chosen bacteria, Imtechella halotolerans is Gram-negative and Allobacillus halotolerans is Gram-positive. Truepera radiovictrix is resistant to lysozyme lysis and has very high GC content. They represent different challenges in NGS-based analysis. Moreover, with accurately quantified cell number and a log abundance distribution, this standard enables absolute cell number quantification in cases such as pathogen load detection.

    Documents

    Technical Specifications

    16S rRNA genes https://s3.amazonaws.com/zymo-files/BioPool/D6321.refseq.zip
    Biosafety This product is not biohazardous as microbes have been fully inactivated.
    Impurity Level < 0.01% foreign microbial DNA
    Sample Storage -80°C
    Storage Solution DNA/RNA Shield™
    Supplemental Info

    Resources

    Studies where strict bioburden is required, such as food or water studies, it would be useful to use the Spike-in Control.

    High biomass samples (feces, cell culture, etc.) should be used with Spike-In I.

    Low Biomass samples (swabs, water filters, etc.) should be used with Spike-In II.

    All bacterial sample types are compatible with the spike-in control.

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