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    ZymoBIOMICS MagBead RNA

    R2137 / R2138


    ZymoBIOMICS MagBead RNA

    Cat # Name Size
    R2137 ZymoBIOMICS MagBead RNA 96 preps
    R2138 ZymoBIOMICS MagBead RNA 4 x 96 preps
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    Highlights
    • Unbiased Lysis: Efficient and unbiased lysis of microbes including gram positive/negative bacteria, fungi, protozoans, and viruses from any sample including feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.
    • Ultra-Pure: High-quality total RNA (including small/microRNAs) is inhibitor-free and ready for RT-PCR and microbiome measurements using Next-Gen Sequencing.
    • High-Sensitivity: Increased detection limit of very low abundance organisms.
    Description

    The ZymoBIOMICS MagBead RNA kit provides a high-throughput, magnetic beadbased purification of total RNA (including small/microRNAs) from any sample source (e.g., microbes including gram positive/negative bacteria, fungi, protozoans, viruses, fecal, soil, plant, water, biofilms, swabs, saliva, body fluids and etc.). The provided DNA/RNA Shield inactivates infectious agents and is ideal for sample storage at ambient temperatures. Total RNA is eluted into ≥50 µl of ZymoBIOMICS DNase/RNase-Free Water and is ready for any downstream application including Next-Gen Sequencing, RT-qPCR, etc.

    For automation scripts and support, email automation@zymoresearch.com

    Binding Capacity 15 µg total RNA per 30 µl MagBinding Beads.
    Equipment Needed Magnetic stand, heat block, liquid handler or robotic sample processor (user provided).
    Purity High-quality RNA is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc.
    Recommended Materials (sold separately) – 96-well Collection Plate (C2002; capacity is up to 1.2 ml/well), 96-Well Block (P1001; capacity is up to 2 ml/well), 96-well Elution Plate (C2003), Cover Foil (C2007), ZR-96 MagStand (P1005).
    Sample Preservation DNA/RNA Shield lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures.
    Sample Types Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA and RNA are efficiently isolated from ≤ 50mg of mammalian feces, ≤ 50mg soil, and 5-20mg (wet weight) of fungal bacterial cells, biofilms, water, and swabs.
    Size Limits Total RNA including small/microRNAs ≥ 17nt.
    Storage RNA eluted with DNase/RNase-Free Water (provided) can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage.
    Supplemental Info

    This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.

    Cat # Name Size
    R1060-2-100 RNA Prep Buffer 100 ml
    R2130-2-80 MagBead DNA/RNA Wash 2 80 ml
    R2130-2-20 MagBead DNA/RNA Wash 2 20 ml
    R2130-1-120 MagBead DNA/RNA Wash 1 120 ml
    R1060-2-50 RNA Prep Buffer 50 ml
    E1010 DNase I Set 250 U
    R1060-1-50
    R1060-1-100
    D4302-6-6 ZymoBIOMICS MagBinding Beads 6 ml
    D4302-6-12 ZymoBIOMICS MagBinding Beads 12 ml
    D4302-5-30 ZymoBIOMICS DNase/RNase Free Water 30 ml
    D4302-5-50 ZymoBIOMICS DNase/RNase Free Water 50 ml
    D4302-5-1 ZymoBIOMICS DNase/RNase Free Water 1 ml
    D4302-5-10 ZymoBIOMICS DNase/RNase Free Water 10 ml
    D3001-2-20 Proteinase K w/ Storage Buffer Set 20 mg
    D3001-2-5 Proteinase K w/ Storage Buffer Set 5 mg
    R1100-50 DNA/RNA Shield 50 ml
    R1100-250 DNA/RNA Shield 250 ml
    S6002-96-3 ZR-96 BashingBead Lysis Rack 0.5 mm & 0.1 mm
    S6012-50 ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) 50 Tubes

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