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DNA/RNA Shield (50 ml)Cat#: R1100-50DNA/RNA Shield reagent is a DNA and RNA stabilization solution for nucleic acids in any biological sample. This DNA and RNA stabilization solution preserves the... -
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Read The Zymo Research PromiseZymoBIOMICS MagBead RNA
| Cat # | Name | Size |
|---|
Highlights
- Unbiased Lysis: Efficient and unbiased lysis of microbes including gram positive/negative bacteria, fungi, protozoans, and viruses from any sample including feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.
- Ultra-Pure: High-quality total RNA (including small/microRNAs) is inhibitor-free and ready for RT-PCR and microbiome measurements using Next-Gen Sequencing.
- High-Sensitivity: Increased detection limit of very low abundance organisms.
Documents
Product Description
Technical Specifications
| Binding Capacity | 15 µg total RNA per 30 µl MagBinding Beads. |
|---|---|
| Equipment Needed | Magnetic stand, heat block, liquid handler or robotic sample processor (user provided). |
| Purity | High-quality RNA is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc. |
| Recommended Materials | (sold separately) – 96-well Collection Plate (C2002; capacity is up to 1.2 ml/well), 96-Well Block (P1001; capacity is up to 2 ml/well), 96-well Elution Plate (C2003), Cover Foil (C2007), ZR-96 MagStand (P1005). |
| Sample Preservation | DNA/RNA Shield lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures. |
| Sample Types | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA and RNA are efficiently isolated from ≤ 50mg of mammalian feces, ≤ 50mg soil, and 5-20mg (wet weight) of fungal bacterial cells, biofilms, water, and swabs. |
| Size Limits | Total RNA including small/microRNAs ≥ 17nt. |
| Storage | RNA eluted with DNase/RNase-Free Water (provided) can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage. |
Resources
Q1: Can I still use DNA/RNA Shield if a precipitate has formed?
This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.
| Cat # | Name | Size | |
|---|---|---|---|
| R1060-2-100 | RNA Prep Buffer | 100 ml | |
| R2130-2-80 | MagBead DNA/RNA Wash 2 | 80 ml | |
| R2130-2-20 | MagBead DNA/RNA Wash 2 | 20 ml | |
| R2130-1-120 | MagBead DNA/RNA Wash 1 | 120 ml | |
| R1060-2-50 | RNA Prep Buffer | 50 ml | |
| E1010 | DNase I Set | 250 U | |
| R1060-1-50 | |||
| R1060-1-100 | |||
| D4302-6-6 | ZymoBIOMICS MagBinding Beads | 6 ml | |
| D4302-6-12 | ZymoBIOMICS MagBinding Beads | 12 ml | |
| D4302-5-30 | ZymoBIOMICS DNase/RNase Free Water | 30 ml | |
| D4302-5-50 | ZymoBIOMICS DNase/RNase Free Water | 50 ml | |
| D4302-5-1 | ZymoBIOMICS DNase/RNase Free Water | 1 ml | |
| D4302-5-10 | ZymoBIOMICS DNase/RNase Free Water | 10 ml | |
| D3001-2-20 | Proteinase K w/ Storage Buffer Set | 20 mg | |
| D3001-2-5 | Proteinase K w/ Storage Buffer Set | 5 mg | |
| R1100-50 | DNA/RNA Shield | 50 ml | |
| R1100-250 | DNA/RNA Shield | 250 ml | |
| S6002-96-3 | ZR-96 BashingBead Lysis Rack | 0.5 mm & 0.1 mm | |
| S6012-50 | ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) | 50 Tubes | |
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