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ZymoBIOMICS MagBead RNA
R2137 / R2138
ZymoBIOMICS MagBead RNA
- Unbiased Lysis: Efficient and unbiased lysis of microbes including gram positive/negative bacteria, fungi, protozoans, and viruses from any sample including feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.
- Ultra-Pure: High-quality total RNA (including small/microRNAs) is inhibitor-free and ready for RT-PCR and microbiome measurements using Next-Gen Sequencing.
- High-Sensitivity: Increased detection limit of very low abundance organisms.
The ZymoBIOMICS MagBead RNA kit provides a high-throughput, magnetic beadbased purification of total RNA (including small/microRNAs) from any sample source (e.g., microbes including gram positive/negative bacteria, fungi, protozoans, viruses, fecal, soil, plant, water, biofilms, swabs, saliva, body fluids and etc.). The provided DNA/RNA Shield inactivates infectious agents and is ideal for sample storage at ambient temperatures. Total RNA is eluted into ≥50 µl of ZymoBIOMICS DNase/RNase-Free Water and is ready for any downstream application including Next-Gen Sequencing, RT-qPCR, etc.
For automation scripts and support, email email@example.com
|Binding Capacity||15 µg total RNA per 30 µl MagBinding Beads.|
|Equipment Needed||Magnetic stand, heat block, liquid handler or robotic sample processor (user provided).|
|Purity||High-quality RNA is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc.|
|Recommended Materials||(sold separately) – 96-well Collection Plate (C2002; capacity is up to 1.2 ml/well), 96-Well Block (P1001; capacity is up to 2 ml/well), 96-well Elution Plate (C2003), Cover Foil (C2007), ZR-96 MagStand (P1005).|
|Sample Preservation||DNA/RNA Shield lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures.|
|Sample Types||Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA and RNA are efficiently isolated from ≤ 50mg of mammalian feces, ≤ 50mg soil, and 5-20mg (wet weight) of fungal bacterial cells, biofilms, water, and swabs.|
|Size Limits||Total RNA including small/microRNAs ≥ 17nt.|
|Storage||RNA eluted with DNase/RNase-Free Water (provided) can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage.|
|R1060-2-100||RNA Prep Buffer||100 ml|
|R2130-2-80||MagBead DNA/RNA Wash 2||80 ml|
|R2130-2-20||MagBead DNA/RNA Wash 2||20 ml|
|R2130-1-120||MagBead DNA/RNA Wash 1||120 ml|
|R1060-2-50||RNA Prep Buffer||50 ml|
|E1010||DNase I Set||250 U|
|D4302-6-6||ZymoBIOMICS MagBinding Beads||6 ml|
|D4302-6-12||ZymoBIOMICS MagBinding Beads||12 ml|
|D4302-5-30||ZymoBIOMICS DNase/RNase Free Water||30 ml|
|D4302-5-50||ZymoBIOMICS DNase/RNase Free Water||50 ml|
|D4302-5-1||ZymoBIOMICS DNase/RNase Free Water||1 ml|
|D4302-5-10||ZymoBIOMICS DNase/RNase Free Water||10 ml|
|D3001-2-20||Proteinase K w/ Storage Buffer Set||20 mg|
|D3001-2-5||Proteinase K w/ Storage Buffer Set||5 mg|
|R1100-50||DNA/RNA Shield||50 ml|
|R1100-250||DNA/RNA Shield||250 ml|
|S6002-96-3||ZR-96 BashingBead Lysis Rack||0.5 mm & 0.1 mm|
|S6012-50||ZR BashingBead Lysis Tubes (0.1 & 0.5 mm)||50 Tubes|